Neurodegeneration Methods and Protocols
Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods a
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Neurodegeneration Methods and Protocols Edited by
Giovanni Manfredi Department of Neurology and Neuroscience, Weill Medical College, Cornell University, New York, NY, USA
Hibiki Kawamata Weill Medical College of Cornell University, New York, NY, USA
Editors Giovanni Manfredi Department of Neurology and Neuroscience Weill Medical College Cornell University New York, NY, USA [email protected]
Hibiki Kawamata Weill Medical College of Cornell University New York, NY, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-327-1 e-ISBN 978-1-61779-328-8 DOI 10.1007/978-1-61779-328-8 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011935542 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease, are becoming a growing burden on our society, in parallel with the aging of the world’s population. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge, and present cutting edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Chapters include protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Each chapter composed in the highly successful Methods in Molecular Biology series format contains introduction of the topic, list of necessary reagents, step-by-step methods, and notes on troubleshooting and avoiding known pitfalls, for reproducible laboratory results. Target audience: Cell biologists, molecular biologists, biochemists, pathologists, and researchers in the field of neurodegeneration. New York, NY, USA New York, NY, USA
Giovanni Manfredi Hibiki Kawamata
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Contents Preface.
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