Oncogene-Induced Senescence Methods and Protocols
This book offers in a single volume a unique collection of the state-of-the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for
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Mikhail A. Nikiforov Editor
OncogeneInduced Senescence Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Oncogene-Induced Senescence Methods and Protocols
Edited by
Mikhail A. Nikiforov Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY, USA
Editor Mikhail A. Nikiforov Department of Cell Stress Biology Roswell Park Cancer Institute Buffalo, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6668-4 ISBN 978-1-4939-6670-7 (eBook) DOI 10.1007/978-1-4939-6670-7 Library of Congress Control Number: 2016955246 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A
Preface Oncogene-induced senescence is a multistep program triggered in response to aberrant oncoprotein expression and/or activation. The eventual function of this fail-safe mechanism is the suppression of the proliferation of cells at the preneoplastic stage ultimately resulting in the prevention of fully malignant progeny. On the other hand, senescent cells have been shown to promote cancer initiation and progression in several mouse models. Since the discovery of oncogene-induced senescence in 1997 by Serrano et al., many outstanding researchers have been working on this intriguing set of phenotypes. In addition to proliferation arrest, cells undergoing oncogene-induced senescence have been initially characterized by changes in the activity of senescence-associated β-galactosidase, cell size, chromatin structure, histone modifications, DNA integrity, etc. During the past two decades,
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