Permeability Barrier Methods and Protocols
The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute tec
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Permeability Barrier Methods and Protocols Edited by
Kursad Turksen Regenerative Medicine Program, Sprott Centre for Stem Cell Research, The Ottawa Hospital Research Institute, Ottawa, ON, Canada
Editor Kursad Turksen, Ph.D Regenerative Medicine Program Sprott Centre for Stem Cell Research The Ottawa Hospital Research Institute Ottawa, ON Canada [email protected]
Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-190-1 e-ISBN 978-1-61779-191-8 DOI 10.1007/978-1-61779-191-8 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011935456 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface When I approached Dr. John Walker, the Editor-in-Chief of the Methods in Molecular Biology series, with the idea of putting together a protocol volume on the permeability barrier, his immediate response was “What a great idea. We have not covered this topic!” John: Thanks for the great support for this volume from the beginning. Although the topic of the permeability barrier is diverse, the authors herein cover many critical areas, making this volume valuable to almost anybody who is interested in this field. I hope that the many valuable protocols included will be useful for both novices and experts alike. I thank all the contributors for very graciously providing their protocols for this volume. Without them and their willingness to share protocol details, this new volume would not have materialized. Patrick Marton, the Editor of the Methods in Molecular Biology series at Springer, has been a great support and is always available to answer my questions and listen to my suggestions. A very special thanks goes to David Casey for his invaluable help during the production stages of this volume. Ottawa, Ontario, Canada
Kursad Turksen
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Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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