Peroxisomes Methods and Protocols

This volume provides easily accessible and comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Chapters detail valuable instructions, guidelines and proto

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Michael Schrader Editor

Peroxisomes Methods and Protocols

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Peroxisomes Methods and Protocols

Edited by

Michael Schrader College of Life and Enivornmental Sciences, Biosciences, University of Exeter, Exeter, UK

Editor Michael Schrader College of Life and Environmental Sciences, Biosciences University of Exeter Exeter, UK

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6935-7    ISBN 978-1-4939-6937-1 (eBook) DOI 10.1007/978-1-4939-6937-1 Library of Congress Control Number: 2017937360 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

This book is dedicated to Tina, Anna and Paula—the “lighthouses” in my life—who make it all possible and to my parents for their ongoing support and interest in peroxisomes.

Preface This edition of Peroxisomes: Methods and Protocols assembles a volume of easily accessible protocols particularly useful for those already working on peroxisomes (and other membrane-bound organelles) as well as for those who would like to start working on this fascinating organelle. Due to their growing importance in health and development, there is increasing interest in the study of peroxisomes. Furthermore, peroxisomes combine properties which render them suitable model organelles to study diverse molecular processes in eukaryotic cells. This edition assembles a comprehensive collection of methods, techniques and s