Plastids Methods and Protocols
This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The ch
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Eric Maréchal Editor
Plastids Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plastids Methods and Protocols
Edited by
Eric Maréchal Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Commissariat à l’Energie Atomique et aux Energies Alternatives, CEA Grenoble, Institut National Recherche Agronomique, UMR5168, Université Grenoble Alpes, Grenoble, France
Editor Eric Maréchal Laboratoire de Physiologie Cellulaire et Végétale Centre National de la Recherche Scientifique Commissariat à l’Energie Atomique et aux Energies Alternatives CEA Grenoble, Institut National Recherche Agronomique UMR5168, Université Grenoble Alpes Grenoble, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8653-8 ISBN 978-1-4939-8654-5 (eBook) https://doi.org/10.1007/978-1-4939-8654-5 Library of Congress Control Number: 2018947772 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Photo credit to Denis Falconet, LPCV, Grenoble Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface A long time ago, on our planet, life has emerged, most likely from an initial prebiotic environment containing self-replicating RNA. Our understanding of the transition to the DNARNA-protein replicative system, characterizing what is meant by living cells and viruses, is poor. Nevertheless, progresses are being made thanks to the expo
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