A new record of Sclerotinia sclerotiorum infecting potato plants in China
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A new record of Sclerotinia sclerotiorum infecting potato plants in China Seyedmohammadreza Ojaghian 1,2 Received: 6 January 2020 / Accepted: 1 April 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Carpogenic . Hangzhou . Koch’s postulates . Myceliogenic . Sclerotia
China is considered to be one of the world’s leading producers of potato. In September 2019, during a plant disease survey in a few potato plantations in Hangzhou (Zhejiang province, East China, GPS coordinates: 30°17'37.14"N, 120°09'41.11"E), water-soaked lesions were observed in the lower parts of potato plants. Black, mature sclerotia, 6–15 mm in diam., appeared within dried beige-coloured lesions. On CDA, after 5–7 days at 21–23 °C, the myceliogenic germination of the sclerotia resulted in colonies consisting of hyaline-white, septate hyphae and globose to elliptical sclerotia. On carrot, carpogenic germination of air-dried sclerotia, previously stored at 4 °C for 55 days, was observed after 45 days at 21–23 °C (Ojaghian 2009). Sixty percent of sclerotia became stipitate and produced funnel-shaped apothecia. The genomic DNA, after extraction and PCR amplification with selective primers SsF (5′-AGTC GAGGGACGGGTACTAA-3′) and SsR (5′-CTTG TCCTCATTGCCGTTT-3′) gave a banding pattern identi-
cal to that of Sclerotina sclerotiorum (Lib.) de Bary from potato in Iran (Ojaghian et al. 2016). The resulting sequence (GenBank accession No. MT080690) of 420 bp was 100% identical to S. sclerotiorum (MN099281, MN105884, MK074848). Pathogenicity of the fungus was tested on potato. Sterilized wheat grains colonized by the fungus were attached with cellophane to the stems of twenty 60-day-old potato plants grown from nondormant tubers. After 5 days at 21–23 °C, white mycelium and sclerotia of S. sclerotiorum appeared on inoculated stems. There were no symptoms on control plants treated with sterile, non-infested wheat grains. To our knowledge, this is the first report of S. sclerotiorum on potato plants in China. Funding information This study was funded by Ton Duc Thang University, Ho Chi Minh City, Vietnam.
References * Seyedmohammadreza Ojaghian [email protected] 1
Smart Agriculture Research and Application Team, Ton Duc Thang University, Ho Chi Minh City, Vietnam
2
Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam
Ojaghian MR (2009) First report of Sclerotinia sclerotiorum on potato plants in Iran. Australas Plant Dis Notes 4:39–41 Ojaghian MR, Zhang JZ, Zhang F, Qiu W, Li XL, Xie G-L, Zhu SJ (2016) Early detection of white mold caused by Sclerotinia sclerotiorum in potato fields using real-time PCR. Mycol Prog 15:959–965 Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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