Abscisic acid-nitric oxide and auxin interaction modulates salt stress response in tomato roots
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Abscisic acid-nitric oxide and auxin interaction modulates salt stress response in tomato roots Mirella Pupo Santos . Daniel Basilio Zandonadi . Ariadne Felicio Lopo de Sa´ . Evenilton Pessoa Costa . Carlos Jorge Logullo de Oliveira . La´zaro E. P. Perez . Arnoldo Rocha Fac¸anha . Ricardo Bressan-Smith
Received: 16 April 2020 / Accepted: 5 September 2020 Ó Brazilian Society of Plant Physiology 2020
Abstract Integrated signaling network involving abscisic acid (ABA), nitric oxide (NO), and indoleacetic acid (IAA) controls root morphogenesis during salt stress by a mechanism still poorly understood. The present data unveiled an ABA-NO-IAA interaction underlying radicular morphological responses to salinity. Three Solanum lycopersicum genotypes were analyzed: wild type, ABA-insensitive mutant (sitiens), and auxin-responsive (DR5::GUS) plants. Nitric oxide fluorescence, nitrate reductase activity, auxin signaling, and some molecular analyses were performed. Pharmacological inhibitors and NO donor sodium nitroprusside were also used to evaluate NaCl-induced root morphological responses. Sodium nitroprusside inhibited primary root length, increased lateral root
emergence, and rescued salt inhibited lateral root growth. The results showed that NO integrates the ABA-IAA signaling network of root system responses under salt stress, involving: (a) ABA and molybdenum-dependent enzymes as responsible for saltinduced NO production; (b) modulations of the plasma membrane H?-ATPase coupling and isoforms differential expression; and (c) ABA-mediated and NOdependent antioxidative enzymes activities. Keywords Salt stress Auxin Nitric oxide Abscisic acid P-ATPase Root morphology
1 Introduction Mirella Pupo Santos, Daniel Basilio Zandonadi, Ariadne Felicio Lopo de Sa´, Evenilton Pessoa Costa, Carlos Jorge Logullo de Oliveira, La´zaro E. P. Perez, Arnoldo Rocha Fac¸anha and Ricardo Bressan-Smith have contributed equally to this paper.
Soil salinization occurs in several regions of the world, mainly in arid and semi-arid regions (Munns and Tester 2008; Wang et al. 2008). Salinized areas are
M. P. Santos (&) D. B. Zandonadi C. J. L. de Oliveira Institute of Biodiversity and Sustainability (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Av. Sa˜o Jose´ Barreto, 764, Sa˜o Jose´ do Barreto, Macae´, RJ 27965-045, Brazil e-mail: [email protected]
E. P. Costa A. R. Fac¸anha Center of Biosciences and Biotechnology, LBCT-CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil
A. F. L. de Sa´ L. E. P. Perez Department of Biological Sciences, Escola Superior de Agricultura ‘‘Luiz de Queiroz’’, Universidade de Sa˜o Paulo, Sa˜o Paulo, Brazil
R. Bressan-Smith Plant Physiology Lab, LMGV-CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil
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Theor. Exp. Plant Physiol.
found in 30% of irrigated soils in the world, and expand 10% per year due to natural and anthropogenic factors tha
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