Adipose-Derived Stem Cells Methods and Protocols
This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in
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Bruce A. Bunnell Jeffrey M. Gimble Editors
AdiposeDerived Stem Cells Methods and Protocols Second Edition
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Adipose-Derived Stem Cells Methods and Protocols Second Edition
Edited by
Bruce A. Bunnell Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA
Jeffrey M. Gimble Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA; LaCell LLC, New Orleans, LA, USA
Editors Bruce A. Bunnell Center for Stem Cell Research and Regenerative Medicine Tulane University School of Medicine New Orleans, LA, USA
Jeffrey M. Gimble Center for Stem Cell Research and Regenerative Medicine Tulane University School of Medicine New Orleans, LA, USA LaCell LLC New Orleans, LA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7797-0 ISBN 978-1-4939-7799-4 (eBook) https://doi.org/10.1007/978-1-4939-7799-4 Library of Congress Control Number: 2018938174 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover caption: Cover image courtesy of Rachel Sabol. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Adipose stromal/stem cells (ASC) remain of intense interest in the field of regenerative medicine for their potent differentiation, immunomodulatory potential, and paracrine actions via their secretome. Over the intervening t
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