Candida pruni sp. nov. is a new yeast species with antagonistic potential against brown rot of peaches
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Candida pruni sp. nov. is a new yeast species with antagonistic potential against brown rot of peaches Dian‑peng Zhang · Cai‑ge Lu · Tao‑tao Zhang · Davide Spadaro · De‑wen Liu · Wei‑cheng Liu
Received: 23 October 2013 / Revised: 19 May 2014 / Accepted: 20 May 2014 / Published online: 8 June 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract Brown rot caused by Monilinia spp. is among the most important postharvest diseases of commercially grown stone fruits, and application of antagonistic yeasts to control brown rot is one promising strategy alternative to chemical fungicides. In this research, new yeast strains were isolated and tested for their activity against peach brown rot caused by Monilinia fructicola. Three yeast strains were originally isolated from the surface of plums (cv Chinese Angelino) collected in the north of China. In artificially wounded inoculation tests, the yeast reduced the brown rot incidence to 20 %. The population of the yeast within inoculated wounds on peaches significantly increased at 25 °C from an initial level of 5.0 × 106 to 4.45 × 107 CFU per wound after 1 day. The antagonistic strains were belonging to a new species of the genus Candida by sequence comparisons of 26 S rDNA D1/D2 domain and internal transcribed spacer region. The strains are most closely related to C. asparagi, C. musae and C. fructus on the basis of the phylogenetic trees based on the D1/D2 region of 26S rDNA. However, the strains are notably different from C. asparagi, C. musae and C. fructus, in morphological and physiological characteristics. Therefore, the name Candida pruni is proposed for the novel species, with sp-Quan (=CBS12814T = KCTC 27526T = GCMC Communicated by: Olaf Kniemeyer. D. Zhang · C. Lu · T. Zhang · D. Liu · W. Liu (*) Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China e-mail: [email protected] D. Spadaro DiSAFA—Department of Agricultural, Forestry and Food Sciences, University of Torino, via L. da Vinci 44, 10095 Grugliasco, TO, Italy
6582T) as the type strain. Our study showed that Candida pruni is a novel yeast species with potential biocontrol against brown rot caused by M. fructicola on peaches. Keywords Candida pruni · Brown rot · Monilinia fructicola · Biological control
Introduction Brown rot caused by Monilinia spp. is among the most important postharvest diseases of commercially grown stone fruits. The most common species isolated from brown rot-infected peaches and nectarines in China, as well as in other countries, is M. fructicola (Tian and Bertolini 1999; Pellegrino et al. 2009), which causes severe losses during the postharvest storage. When permitted, applying synthetic fungicides is the best method to control the postharvest diseases on fruits. However, consumers are increasingly concerned about safety and prefer food free of pesticide residues, toxins and harmful microorganisms (Spadaro and Gullino 2004). Moreover, many fungal strains are developing resistance to widely
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