Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis , a squat-lobster from the southwestern Pa
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TECHNICAL NOTE
Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific Emily A. Boyle • Andrew David Thaler Alixandra Jacobson • Sophie Plouviez • Cindy Lee Van Dover
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Received: 22 January 2013 / Accepted: 24 January 2013 / Published online: 3 February 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Munidopsis lauensis is a deep-sea squat lobster commonly associated with hydrothermal vents at southwest Pacific back-arc spreading centers. Eight selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci from a related species were successfully cross-amplified. Eight of the ten total loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.23 to 0.64 (mean HO = 0.50, SD = 0.15). Seven of ten loci crossamplified in Munidopsis antonii, a closely related species. Microsatellites developed for M. lauensis are being deployed to study connectivity among populations of this species that occur at hydrothermal vents in Manus and Lau Basins. Keywords Munidopsis antonii Hydrothermal vent Back-arc basin Manus Basin
Deep-sea hydrothermal vents are patchy, ephemeral habitats that support biomass-rich communities. Assessing genetic structure among fauna commonly associated with these communities provides insight into patterns of dispersal, connectivity, and gene flow in deep-sea species (e.g., Vrijenhoek 1997, 2010). While there are numerous studies examining the population structure of vent-endemic species from the southwestern Pacific (see Vrijenhoek 2010),
E. A. Boyle A. D. Thaler (&) A. Jacobson S. Plouviez C. L. Van Dover Marine Laboratory, Nicholas School of the Environment, Duke University, 135 Duke Marine Lab Road, Beaufort, NC 28516, USA e-mail: [email protected]; [email protected]
relatively few studies have focused on vent-associated vagrant species whose distribution is not exclusively limited to hydrothermal vents. Assessing genetic structure in vagrant deep-sea species can help distinguish between patterns of population structure specific to hydrothermal vent endemic populations and general patterns in the deep sea. Munidopsis lauensis is a squat lobster commonly associated with, but not endemic to, deep-sea hydrothermal vents of southwestern Pacific back-arc spreading centers (Baba and de Saint Laurent 1992). This paper describes 10 microsatellite markers developed to assess genetic structure among populations of M. lauensis in the southwest Pacific. Munidopsis lauensis genomic DNA was extracted from ethanol preserved abdominal muscle tissue using Wizard SV Genomic DNA Extraction Kit following protocols provided by the manufacturer (Promega, Madison, WI, USA). Eight microsatellite loci developed for Munidopsis polymorpha (Cabezas et al. 2009) and three loci developed for Munida rugosa and Munida sarsi (Bailie et al. 2011) were tested for cross-amplification in M. lauensis. Two of these loci cross-amplif
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