Ectopic overexpression of bol-miR390a from broccoli ( B . oleracea L var. italica ) increases lateral branches in Arabid
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ORIGINAL PAPER
Ectopic overexpression of bol‑miR390a from broccoli (B. oleracea L var. italica) increases lateral branches in Arabidopsis Lixia He1 · Yu Wang1 · Yinglan Jia1 · Yaling Yang2 · Xue Han1 · Jiye Yuan1 · Lihong Li1 · Peng Li3 · Chengbin Chen1 · Wenqin Song1 · Min Liu4 · Hui Li2 · Chunguo Wang1 Received: 5 August 2019 / Accepted: 29 August 2020 © Springer Nature B.V. 2020
Abstract MiR390 is a conserved miRNA family and that has been confirmed to play pleiotropic roles in regulating the growth and development of plant species. Broccoli (B. oleracea L var. italica) is an important varietas of B. oleracea with abundant nutrients and cultivated worldwide. However, the mechanism underlying the miR390-mediated regulation of organ development in broccoli remains unknown. In the present study, bol-miR390a, a member of miR390 family, was identified in broccoli. Bol-miR390a displayed significantly differential expression patterns with the development of various broccoli organs. Ectopic overexpression of bol-miR390a accelerated the lateral organ development of transgenic lines in Arabidopsis. The lateral branches and roots of overexpressed bol-miR390a transgenic Arabidopsis were greater than those of the vector controls; thus, the biomass of 35S::bol-miR390a plants significantly increased relative to those of the vector controls. Moreover, the present results demonstrated that the expression levels of TRANS-ACTING SIRNA3 (TAS3)-derived siRNAs (tasiRNAs) displayed positive correlation with those of the bol-miR390a, whereas TAS3 and several auxin response factors (ARFs) including ARF2, ARF3, and ARF4, which were the potential targets of tasiRNAs, showed decreased expression trends in the overexpressed bol-miR390a transgenic plants compared with those in the vector controls. These results suggested that the bol-miR390a/TAS3/ARFs module might play important roles in the lateral organ development of broccoli. In summary, these findings confirmed that bol-miR390a is a crucial regulator in the lateral development of broccoli and suggested the potential application of bol-miR390a in breeding high-biomass crops and economic plants. Keywords Broccoli (B. oleracea L var. italica) · Bol-miR390a · Lateral branch · TAS3 · ARF
Introduction MicroRNAs (miRNAs) are ~ 22-nucleotide endogenous small RNAs known to post-transcriptionally repress gene expression by mRNA cleavage or translational repression in animals, plants, and some viruses (Bartel 2004; Baskerville and Bartel 2005; Berezikov and Plasterk 2005; Cullen 2006; Mallory and Vaucheret 2006). The first two endogenous miRNAs to be identified were lin-4 and let-7 in C. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00657-6) contains supplementary material, which is available to authorized users. * Hui Li [email protected] * Chunguo Wang [email protected] Extended author information available on the last page of the article
elegans, both of which are key regulators in the pathway controlling the timing of larva
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