Halomonas marinisediminis sp. nov., A Moderately Halophilic Bacterium Isolated from the Bohai Gulf
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Halomonas marinisediminis sp. nov., A Moderately Halophilic Bacterium Isolated from the Bohai Gulf Huilin Zhao1 · Chaonan Wang2 · Yue Tian1 · Yanli Zhang1 · Yulong Wu1 · Yan Zhang3 · Zongjun Du2,4 · Boqing Li1 · Xiaofei Ji1 Received: 15 June 2019 / Accepted: 25 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel Gram-stain-negative, moderately halophilic bacterium, designated strain 204T, was isolated from a marine sediment sample in the Bohai Gulf, Yellow Sea, China. Cells of strain 204T are aerobic, motile, cocci or short rods with two lateral flagella. Growth was observed at 15–40 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, 7.0–7.5) and in the presence of 1.0–18.0% (w/v) NaCl (optimum, 3.0–8.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 204T belonged to the genus Halomonas, showing highest sequence similarity to Halomonas alimentaria YKJ-16T (98.08%), followed by Halomonas sediminicola (97.47%), Halomonas fontilapidosi (97.14%), Halomonas halodenitrificans (96.98%), Halomonas ventosae (96.92%), and Halomonas shengliensis (96.85%). The draft genome is 3.8 Mb in length, containing 3673 protein-coding genes, 62 tRNA genes and 10 rRNA genes, and DNA G+C content is 62.7 mol%. Strain 204T contains phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol as the main polar lipids, and the predominant respiratory quinone was ubiquinone Q-9. The major cellular fatty acids (> 5%) are C 18:1ω7c, C16:1ω7c and/ or C16:1ω6c, C16:0 and C 12:03-OH. Strain 2 04T was clearly distinguished from the closely related type strains through phylogenetic analysis, dDNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics comparisons. On the basis of the polyphasic analysis, strain 2 04T is considered to represent a novel species of the genus Halomonas, for which the name Halomonas marinisediminis sp. nov. is proposed. The type strain is 204T (= MCCC 1H00366T = KCTC 62957T).
Introduction The GenBank accession numbers of Halomonas marinisediminis 204T are MK347065 (16S rRNA gene) and SLTR00000000 (draft genome), respectively. The Digital Protologue database (DPD) number for strain 204T is TA00990 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02147-4) contains supplementary material, which is available to authorized users. * Boqing Li [email protected] * Xiaofei Ji [email protected] 1
Department of Pathogenic Biology, School of Basic Medical Sciences, Binzhou Medical University, Yantai, China
2
College of Marine Science, Shandong University at Weihai, Weihai, China
3
School of Pharmacy, Binzhou Medical University, Yantai, China
4
State Key Laboratory of Microbial Technology, Shandong University, Jinan, China
The genus Halomonas, first proposed by Vreeland et al. [1], belongs to the family Halomonadaceae [2]. Apart from Halomonas, the family comprises of fourteen different genera (https://www.
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