Intestinal Microbiota Composition in Iranian Diabetic, Pre-diabetic and Healthy Individuals
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RESEARCH ARTICLE
Intestinal Microbiota Composition in Iranian Diabetic, Pre-diabetic and Healthy Individuals Farahnaz Ghaemi 1 & Abolfazl Fateh 2,3 & Abbas Akhavan Sepahy 4 & Mehrangiz Zangeneh 5 & Mostafa Ghanei 6 & Seyed Davar Siadat 2,3,7 Received: 18 April 2020 / Accepted: 25 August 2020 # Springer Nature Switzerland AG 2020
Abstract Background Type 2 diabetes, as the most prevalent metabolic disorder, is one of the leading causes of death worldwide. Recent studies showed a significant association between intestinal microbiota and type 2 diabetes. These studies have shared evidences that alteration in the composition of intestinal microbiota can disrupt the balance of the host homeostasis and lead to metabolic disorders such as type 2 diabetes. In the present study, we compared the intestinal microbiota composition in three groups of type 2 diabetes patients, pre-diabetic patients and healthy individuals of Iranian population. Methods After obtaining informed consent, stool samples were collected from 90 individuals of three studied groups. The DNA was extracted using column-based method. Intestinal microbiota composition was evaluated by quantitative real-time PCR using specific bacterial 16S rRNA primers. The difference of bacterial load was compared between three groups. Results The prevalence of Akkermansia muciniphila and Bifidobacteria species in healthy group was higher than type 2 diabetes group (P Value 0.006 and 0.001, respectively). In contrast, the load of Lactobacillus (P Value 0.044), Escherichia coli (P Value 0.005), and Bacteroides fragilis (P Value 0.017) in type 2 diabetes group, and the frequency of E. coli (P Value 0.001) and Bacteroides fragilis (P Value 0.004) in pre-diabetic group was significantly higher than healthy group. Moreover, the frequency of Faecalibacterium prausnitzii in healthy group was significantly higher compared to two other groups (P Value 0.005). Conclusion There is a correlation between intestinal microbiota composition and type 2 diabetes. Determination and restoration of this microbiota composition pattern may have a possible role in prevention and control of type 2 diabetes in a certain population. Keywords microbiota . gut . type 2 diabetes . pre-diabetes
Background * Seyed Davar Siadat [email protected] 1
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2
Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, No. 358, 12th Farvardin Ave, Jomhhoori St, Tehran 1316943551, Iran
3
Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
4
Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran
5
Department of Infectious Diseases, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
6
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
7
Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University
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