Microbial Lipid Production Methods and Protocols

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agric

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Venkatesh Balan Editor

Microbial Lipid Production Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Microbial Lipid Production Methods and Protocols

Edited by

Venkatesh Balan Department of Engineering Technology, Biotechnology Program, College of Technology, University of Houston, Houston, TX, USA

Editor Venkatesh Balan Department of Engineering Technology Biotechnology Program College of Technology University of Houston Houston, TX, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9483-0 ISBN 978-1-4939-9484-7 (eBook) https://doi.org/10.1007/978-1-4939-9484-7 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Lipids derived from conventional sources such as oil seeds include building blocks of cellular membranes. They are utilized to make substances used as energy storage, insulation, a method of cellular communication, and protection. With population growth and increasingly limited farmland, there is an ever-growing demand to develop alternate sources of lipids to meet our food and energy needs. Oleaginous microorganisms such as bacteria, algae, yeast, fungi, and thraustochytrids are a promising, largely untapped, resource for lipid production. Microbial lipids offer some unique advantages, such as high content of unique polyunsaturated fatty acids that are widely used for dietary purposes and of chemically modified lipids that confer desirab