MicroRNA Profiling Methods and Protocols
This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and
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Sweta Rani Editor
MicroRNA Profiling Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
MicroRNA Profiling Methods and Protocols
Edited by
Sweta Rani Department of Science, Waterford Institute of Technology, Waterford, Ireland
Editor Sweta Rani Department of Science Waterford Institute of Technology Waterford, Ireland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6522-9 ISBN 978-1-4939-6524-3 (eBook) DOI 10.1007/978-1-4939-6524-3 Library of Congress Control Number: 2016953512 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC
Preface MicroRNAs (miRNAs) are a small non-coding, single-stranded RNA molecule consisting of 20–24 nucleotides. It is found in animals and plants as well as some viruses and is involved in the regulation of gene expression. The primary function of miRNA is, at the post-transcriptional level, downregulating gene expression by regulating translational repression, mRNA cleavage, and deadenylation. Since the discovery of miRNAs in 1993, this field has garnered major interests among the scientists worldwide. This volume of Methods in Molecular Biology book collates chapters by the experts in their respective field. This book not only provides classical techniques to profile miRNA but also includes newer approaches. The chapters include step-by-step easy-to-follow protocol and troubleshooting tips to ensure successful experiment. To summarize, this book includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters describe the effect of
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