Plant Microtechniques and Protocols
This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especial
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Edward Chee Tak Yeung • Claudio Stasolla Michael John Sumner • Bing Quan Huang Editors
Plant Microtechniques and Protocols
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Editors Edward Chee Tak Yeung Department of Biological Sciences University of Calgary Calgary Alberta Canada Claudio Stasolla Department of Plant Sciences University of Manitoba Winnipeg Manitoba Canada
Michael John Sumner Department of Biological Sciences University of Manitoba Winnipeg Manitoba Canada Bing Quan Huang Center for Basic Research in Digestive Disease Mayo Clinic Rochester Minnesota USA
ISBN 978-3-319-19943-6 ISBN 978-3-319-19944-3 (eBook) DOI 10.1007/978-3-319-19944-3 Library of Congress Control Number: 2015949269 Springer Cham Heidelberg New York Dordrecht London © Springer International Publishing Switzerland 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Springer International Publishing AG Switzerland is part of Science+Business Media (www.springer.com)
Preface
Old and new techniques are essential for any research program. New instrumentation, techniques, methodologies, and ideas continue to appear in the literature. Some will have a profound influence on our future research activities. At present, many traditional methods are still routinely in use in many laboratories, and simple methods such as hand sections and related techniques are extremely useful in botanical research. It is unfortunate, however, that many traditional methods are not being taught. Students often lack a clear understanding of the methods used and, therefore, cannot take full advantage of the different microtechniques available for their experimental studies. The usefulness of many histological methods is that by providing basic structural information, they help in generating questions and hypotheses needed to advance research. The purpose of putting together a volume related to plant microtechniques is to gather the commonly used methods and update their procedures using a simple and fully understandable appr
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