Proteomic and physiological analyses reveal detoxification and antioxidation induced by Cd stress in Kandelia candel roo
- PDF / 629,594 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 185 Views
ORIGINAL PAPER
Proteomic and physiological analyses reveal detoxification and antioxidation induced by Cd stress in Kandelia candel roots Zhao-Xia Weng • Ling-Xia Wang • Fang-lin Tan Li Huang • Jian-Hong Xing • Shi-Pin Chen • Chi-Lien Cheng • Wei Chen
•
Received: 4 July 2012 / Revised: 24 September 2012 / Accepted: 14 November 2012 / Published online: 4 December 2012 Ó Springer-Verlag Berlin Heidelberg 2012
Abstract The heavy metal Cadmium (Cd), added to the water bodies through weathering of rocks and human activities, constitutes one of the major environmental pollutants toxic to plants. This study examines the proteome changes in roots of actively growing Kandelia candel (L.) Druce when challenged with Cd. This mangrove-like species proliferates in estuaries and bays and is a potential choice for phytoremediation of Cd. A total of 53 proteins were up- or down-regulated following a short-term Cd treatment. The identities of the differentially expressed proteins were
determined by MALDI-TOF/TOF. Approximately half of the up-regulated proteins are involved in oxidative response, including antioxidant enzymes, enzymes required for glutathione biosynthesis, enzymes in TCA and PPP cycles for generating ATP, NADH and NADPH. These results support the prediction that a prompt antioxidative response is necessary for the reduction of the oxidative stress caused by Cd and set the stage for further investigating of Cd up-regulated proteins in Kandelia candel. Keywords Cadmium Proteomic analysis Physiological response Kandelia candel
Z.-X. Weng and L.-X. Wang contributed equally. Communicated by H. Rennenberg.
Introduction
Electronic supplementary material The online version of this article (doi:10.1007/s00468-012-0811-7) contains supplementary material, which is available to authorized users.
Cadmium (Cd) is not biodegradable. As a consequence, once released into the environment, it persists as a major contaminant. In plants, severe Cd exposure induces various symptoms of phytotoxicity including chlorosis, reduction of biomass, inhibition of root elongation and the eventual death (Milone et al. 2003). Cd is a bivalent cation that causes the exposed plants to produce reactive oxygen species (ROS) (Cuypers et al. 2010). Hydrogen peroxide (H2O2), superoxide (O- 2 ) and hydroxyl (OH ) radicals are among the most reactive compounds produced during heavy metal stress (Dazy et al. 2009). Accumulation of ROS can result in cell death due to oxidative processes such as membrane lipid peroxidation, protein oxidation, inhibition of enzyme activity, and DNA and RNA damage (Mittler 2002). In order to protect themselves from the damage of ROS, plants have evolved an anti-ROS system to fight against these free radicals including those generated in response to heavy metals. The anti-ROS defense system comprises both enzymatic and non-enzymatic
Z.-X. Weng L.-X. Wang J.-H. Xing W. Chen Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Corps, Fujian Agriculture and Forestry Univers
Data Loading...
