Sequential expression of key genes in proline, glycine betaine and artemisinin biosynthesis of Artemisia aucheri Boiss u
- PDF / 1,568,909 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 14 Downloads / 159 Views
ORIGINAL ARTICLE
Sequential expression of key genes in proline, glycine betaine and artemisinin biosynthesis of Artemisia aucheri Boiss using salicylic acid under in vitro osmotic stress Jalil Abbaspour 1 & Ali Akbar Ehsanpour 1 Received: 7 October 2019 / Accepted: 30 April 2020 # Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2020
Abstract Artemisia aucheri belongs to the Asteraceae family, which is known for its medicinal and aromatic properties. The present study was conducted to elucidate the influence of salicylic acid (SA) pretreatment on proline and glycine betaine (GB) metabolism as well as artemisinin biosynthesis in A. aucheri under in vitro osmotic stress ( 0.6 MPa) induced by polyethylene glycol (PEG). The expression of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and betaine aldehyde dehydrogenase (BADH) genes and related enzymes were enhanced under osmotic stress. SA pretreatment improved accumulation of proline and GB and increased the expression of BADH and P5CS enzyme activity under osmotic stress. However, proline dehydrogenase (PDH) gene expression and PDH activity were not affected by osmotic stress and SA pretreatments. In addition, SA in combination with or without PEG induced expression of the key genes involved in artemisinin biosynthesis and increased artemisinin content. These findings indicated that, higher accumulation of proline and GB under osmotic stress by SA is possibly due to increasing of osmoprotectants biosynthesis. This study suggested that A. aucheri is one of the potential species for artemisinin biosynthesis under environmental stress and exogenous SA. Keywords Artemisia aucheri. Gene expression. Osmotic stress. Salicylic acid
Abbreviations ADS BADH CMO CYP71AV1 GB MS PAL P5C P5CDH P5CS PDH PEG
Amorpha-4,11-diene synthase Betaine aldehyde dehydrogenase Choline monooxygenase Cytochrome P450 monooxygenase Glycine betaine Murashige and Skoog Phenylalanine ammonia lyase Δ1-pyrroline-5-carboxylate Proline 5-carboxylate dehydrogenase Δ1-pyrroline-5-carboxylate synthetase Proline dehydrogenase Polyethylene glycol
* Ali Akbar Ehsanpour [email protected] Jalil Abbaspour [email protected] 1
Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
ROS SA
Reaction oxygen species Salicylic acid
Introduction Worldwide drought, which results in osmotic stress, is one of the most challenging threats to plant productivity. Due to induction of numerous adverse effects in biochemical, physiological and molecular processes of plants, osmotic stress causes severe reduction in plant growth, development and productivity (Hasanuzzaman et al. 2013). To survive such unfavorable environmental conditions, plants have developed various protective mechanisms that allow them to adapt to the harsh environment. The synthesis and accumulation of organic solutes, known as osmoprotectants, or compatible solutes such as proline and glycine betaine is one of the striking metabolic responses of plants to
Data Loading...
