Pedobacter puniceum sp. nov. Isolated from Sludge
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Pedobacter puniceum sp. nov. Isolated from Sludge Zhou Yang1 · Jianyi Xu1 · Mengyao Sheng1 · Jiguo Qiu1 · Jianchun Zhu2 · Jun Zhang3 · Jian He1 Received: 19 March 2020 / Accepted: 1 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Strain HX-22-1T was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical factory in Nanjing city, Jiangsu province, PR China. Strain HX-22-1T is Gram-staining-negative, rod-shaped, non-spore-forming and non-flagellated. The 16S rRNA gene-based phylogenetic analysis indicates that the strain HX-22-1T belongs to the genus Pedobacter, closely related to Pedobacter glucosidilyticus KCTC 22438T (98.63% similarity). The genomic DNA G+C content of the strain was 34.4 mol%. Strain HX-22-1T was able to grow at 16–37 °C (optimum at 30 °C), at pH 6.0–8.0 (optimum at pH 7.0), and with 0–1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents 16:1ω6c). The predominant respiratory ubiquinone was MK-7. were iso-C15:0 and summed feature 3 (iso-C16:1ω7c and/or C The polar lipid profile is composed of phosphatidylethanolamine (PE), aminophospholipid (APL), aminolipid (AL), and phospholipids (PL). The ANI and dDDH values obtained between the genomes of HX-22-1T and P. glucosidilyticus KCTC 22438T were 89.6 and 38.8%, respectively. On the basis of data from phenotypic, chemotaxonomic, and genotypic analysis, strain HX-22-1T represents a novel species of the genus Pedobacter, for which the name Pedobacter puniceum sp. nov. is proposed. The type strain is HX-22-1T (= KCTC 72655T = CCTCC AB 2019348T).
Introduction The genus Pedobacter was proposed early by Steyn et al. [1]. Members of this genus are Gram-staining-negative, rodshaped, and non-spore-forming, with MK-7 as major respiratory quinone and iso-C15:0 as the main fatty acid components [2]. At the time of writing, the genus contained 79 species (https://www.bacterio.net/pedobacter.html). Type strains of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02235-5) contains supplementary material, which is available to authorized users. * Jun Zhang [email protected] * Jian He [email protected] 1
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
2
Laboratory Centre of Life Science, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
3
The College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
genus Pedobacter have been isolated from various environments such as soil, sediment, lake, river, compost, alpine glacier cryoconite, ancient tree trunk, hard-water rivulet, glacier, falls, rhizosphere, chilled food, fragmentary rock, activated sludge, maize root, and vanadium mine. In this study, we report on the polyphasic study of a Pedobacterlik
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