Neptunomonas marina sp. nov., isolated from seawater
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ORIGINAL PAPER
Neptunomonas marina sp. nov., isolated from seawater Wen‑Ming Chen1 · Tsai‑Ying Hsieh1 · Shih‑Yi Sheu2 Received: 14 March 2019 / Revised: 25 April 2019 / Accepted: 6 May 2019 / Published online: 21 May 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract Strain HPM-16T, isolated from seawater, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of an up-to-date bacterial core gene set (92 protein clusters) indicated that strain HPM-16T formed a phylogenetic lineage in the genus Neptunomonas. Strain HPM-16T was most closely related to Neptunomonas concharum LHW37T with 16S rRNA gene sequence similarity of 96.7%. Cells were Gram-stain negative, facultatively anaerobic, motile by means of a single polar flagellum, rod-shaped and formed white colonies. Optimal growth occurred at 30–35 °C, pH 6.5–8, and in the presence of 2–5% NaCl. C 18:1ω7c and summed feature 3 ( C16:1ω7c and/or C16:1ω6c) were the predominant fatty acids. The only isoprenoid quinone was Q-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and several uncharacterized lipids. The major polyamines were putrescine and spermidine. The draft genome was approximately 3.68 Mb in size with a G + C content of 50.5 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain HPM-16T should be classified as a novel species of the genus Neptunomonas, for which the name Neptunomonas marina sp. nov. is presented. The type strain is HPM-16T (= BCRC 80980T = LMG 29560T = KCTC 52235T). Keywords Neptunomonas marina sp. nov. · Gammaproteobacteria · Oceanospirillales · Oceanospirillaceae · Polyphasic taxonomy
Introduction
Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and the draft genome of Neptunomonas marina strain HPM-16T are LT223128 and SACQ00000000. The Digital Protologue database Taxon Number for strain HPM-16T is TA00504. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-019-01671-7) contains supplementary material, which is available to authorized users. * Shih‑Yi Sheu [email protected] 1
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai‑Chuan Rd. Nan‑Tzu, Kaohsiung City 811, Taiwan
Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai‑Chuan Rd. Nan‑Tzu, Kaohsiung City 811, Taiwan
2
The genus Neptunomonas, first established by Hedlund et al. (1999) with type species Neptunomonas naphthovorans and emended by Lee et al. (2012) and Yang et al. (2014), is a member of the family Oceanospirillaceae, in the order Oceanospirillales of the class Gammaproteobacteria. At the time of writing, this genus comprises seven recognized species according to the List of Prokaryotic names with Standing in Nomenc
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