Streptococcus pneumoniae Methods and Protocols
This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of
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Federico Iovino Editor
Streptococcus pneumoniae Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Streptococcus pneumoniae Methods and Protocols
Edited by
Federico Iovino Department of Microbiology, Tumor and Cell Biology Karolinska Institutet, Bioclinicum, Stockholm, Sweden Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
Editor Federico Iovino Department of Microbiology, Tumor and Cell Biology Karolinska Institutet, Bioclinicum Stockholm, Sweden Department of Clinical Microbiology Karolinska University Hospital Stockholm, Sweden
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9198-3 ISBN 978-1-4939-9199-0 (eBook) https://doi.org/10.1007/978-1-4939-9199-0 Library of Congress Control Number: 2019934805 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Aim of the Book Abstract The Gram-positive bacterium Streptococcus pneumoniae, the pneumococcus, is a leading cause of mortality and morbidity worldwide and considered a serious threat in today’s public health. It is a major contributor of severe diseases such as pneumonia and bacteremia and the main etiological cause of bacterial meningitis. All these diseases are defined as invasive pneumococcal disease (IPD). Even though pneumococci can cause invasive diseases, S. pneumoniae is a commensal, and in fact, it normally colonizes the nasopharyngeal epithelium asympto
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