Microlunatus cavernae sp. nov., a novel actinobacterium isolated from Alu ancient cave, Yunnan, south-west China
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ORIGINAL PAPER
Microlunatus cavernae sp. nov., a novel actinobacterium isolated from Alu ancient cave, Yunnan, south-west China Juan Cheng • Wei Chen • Bing Huo-Zhang • Salam Nimaichand • En-Min Zhou • Xin-Hua Lu • Hans-Peter Klenk • Wen-Jun Li
Received: 20 February 2013 / Accepted: 29 April 2013 / Published online: 8 May 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract A Gram-positive, coccoid, non-endospore-forming actinobacterium, designated YIM C01117T, was isolated from a soil sample collected from Alu ancient cave, Yunnan province, south-west China. Based on the 16S rRNA gene sequence analysis, strain YIM C01117T was shown to belong to the genus Microlunatus, with highest sequence similarity of 97.4 % to Microlunatus soli DSM 21800T. The whole genomic DNA relatedness as shown by the DNA– DNA hybridization study between YIM C01117T and
Juan Cheng and Wei Chen contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s10482-013-9929-x) contains supplementary material, which is available to authorized users. J. Cheng B. Huo-Zhang S. Nimaichand E.-M. Zhou W.-J. Li (&) Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People’s Republic of China e-mail: [email protected]; [email protected] W. Chen Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming 650202, People’s Republic of China S. Nimaichand Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal 795003, Manipur, India
M. soli DSM 21800T had a low value (47 ± 2 %). Strain YIM C01117T was determined to contain LLdiaminopimelic acid with Gly, Glu and Ala amino acids (A3c0 type) in the cell wall. Whole-cell hydrolysates were found to contain glucose, galactose, mannose and ribose. The major polar lipids were determined to be phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone system present is MK-9(H4), while the major fatty acids were identified to be anteiso-C15:0 (24.1 %), iso-C16:0 (22.3 %) and iso-C15:0 (11.4 %). The G?C content of the genomic DNA was determined to be 65.9 mol%. The chemotaxonomic and genotypic data support the affiliation of the strain YIM C01117T to the genus Microlunatus. The results of physiological and biochemical tests allow strain YIM C01117T to be X.-H. Lu The National Engineering Center for Research of Microbial Pharmaceuticals and New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Shijiazhuang 050015, People’s Republic of China H.-P. Klenk Leibniz Institute—DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, Germany W.-J. Li Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, ¨ ru¨mqi 830011, People’s Chinese Academy of Sciences, U Republic of China
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