Psychroflexus maritimus sp. nov., isolated from coastal sediment
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ORIGINAL PAPER
Psychroflexus maritimus sp. nov., isolated from coastal sediment Yan‑Lin Zhong1 · Rui Zhang1 · Xiao‑Yu Zhang1 · Li‑Xue Yu1 · Meng‑Fan Zhao1 · Zong‑Jun Du1,2 Received: 10 March 2020 / Revised: 30 April 2020 / Accepted: 30 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped bacterium, designated C 1T was isolated from the coastal sediment of Xiaoshi Island, Weihai, China. Phylogenetic analysis of the 16S rRNA gene and the genome sequence of the newly isolated strain revealed that it belonged to the genus Psychroflexus within the family Flavobacteriaceae. The 16S rRNA gene sequence similarities between strain C1T and the type strains of Psychroflexus members ranged from 91.6 to 94.9%. The AAI, POCP, ANI and dDDH values between strain C 1T and Psychroflexus torquis ATCC 7 00755T were 67.0%, 53.1%, 70.5% and 20.2%, respectively. The DNA G+C content was 34.0 mol%. Strain C1T grows optimally at 28–30 ℃, at pH 7.5–8.0 and with 3.0–5.0% (w/v) NaCl, and its colonies were orange-colored, convex and circular on the MA plate. Positive for hydrolysis of Tween 20 and catalase activities. The dominant respiratory quinone was menaquinone-6, and the major fatty acids were iso-C17:0 3-OH and iso-C15:0. The polar lipids of strain C1T consisted of phosphatidylethanolamine and three unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic data, it is considered that strain C1T represents a novel species within the genus Psychroflexus, for which, the name Psychroflexus maritimus sp. nov., is proposed. The type strain is C1T (= MCCC 1H00415T = KCTC 72796T). Keywords Psychroflexus maritimus · Flavobacteriaceae · 16S rRNA gene · Draft genome sequencing · Polyphasic taxonomy
Introduction
The GenBank accession number for the 16S rRNA gene sequence of strain C1T is MN689620. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAANAS000000000 and the version described in this paper is version JAANAS010000000.
type species. By February 2020, the genus Psychroflexus contained eleven validly published species (https://www. bacterio.net/psychroflexus.html), most of which have been isolated from various saline environments, including sea ice (Bowman et al. 1998), salt lake (Zhong et al. 2016; Donachie et al. 2004; Chen et al. 2009), tidal flat (Park et al. 2016), salt pan (Chun et al. 2014) and marine solar saltern (Jin et al. 2016; Yoon et al. 2009). Members of the genus Psychroflexus have been shown to be present on cheese and believed to play an important role in cheese ripening (Schmitz-Esser et al. 2018; Delcenserie et al. 2014). In the present study, we characterized a representative of a novel species of the genus Psychroflexus.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01933-9) contains supplementary material, which is available to authorized users.
Materials and metho
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