Structure and predictive metabolic contribution of intestinal microbiota of Longfin yellowtail ( Seriola rivoliana ) juv
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ORIGINAL ARTICLE
Structure and predictive metabolic contribution of intestinal microbiota of Longfin yellowtail (Seriola rivoliana) juveniles in aquaculture systems Joan Salas‑Leiva1 · José Manuel Mazón‑Suástegui2 · Andressa Teles2 · Dariel Tovar‑Ramírez2 Received: 12 June 2020 / Accepted: 2 November 2020 © Springer Nature B.V. 2020
Abstract Seriola rivoliana intestinal microbiota (IM) was characterised under aquaculture conditions through 16S rRNA amplicon sequencing. Specimens of 30 days after hatching (DAH) were maintained in three tanks and fed under the same environmental conditions for characterisation 15 days prior to sampling. Three fish were randomly taken from each tank; total DNA extraction of the gut microbiota was performed to characterise microbial composition and its metabolic prediction. The V3 hypervariable region of the 16S rRNA was amplified and sequenced with Illumina pair-end technology. The prokaryotic components in the S. rivoliana intestine were dominated mainly by the phyla Proteobacteria, Firmicutes, Bacteroidetes, Cyanobacteria and Actinobacteria. No significant differences in beta diversity were detected in the three samples (tanks). However in alpha diversity, they were detected in juveniles of the same cohort within the same group, as exemplified by enrichment of certain bacterial groups, mainly of the Clostridia class, which were specific in each fish within the same tank. The metabolic prediction analyses suggested that S. rivoliana IM contribute to the metabolism of amino acids, carbohydrates, lipids, and immune system. This study provides the first IM characterisation under rearing conditions of S. rivoliana—a species with broad economic potential—and contributes to novel information for potential use of probiotics in future trials. Keywords Community ecology · Gut bacteria · Metabolic functions · Seriola rivoliana · 16S rRNA
Introduction Longfin yellowtail Seriola rivoliana (Carangidae) is considered a prospect for intensive aquaculture worldwide because of its rapid growth, high meat quality and adaptability of juveniles to intensive farming conditions [1, 2]. The genus Seriola are carnivorous fish farmed mainly in cages and fed with fresh fish or granulated diets containing high levels Joan Salas-Leiva and José Manuel Mazón-Suástegui are equally contributing first co-author. * Dariel Tovar‑Ramírez [email protected] 1
Consejo Nacional de Ciencia y Tecnología (CONACyT), Centro de Investigación en Materiales Avanzados (CIMAV), Miguel de Cervantes 120, Complejo Industrial Chihuahua, C.P. 31136 Chihuahua, CHIH, Mexico
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. IPN 195, Col Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, BCS, Mexico
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of fishmeal and fish oil [1]. The availability of high quality juveniles, which guarantees a commercial scale culture, has been limited by high mortalities in early rearing phases (larviculture and weaning) [3–5]. Several studies have provided basic information on broodstock management and spawning, ontogenetic diges
