T-Cell Differentiation Methods and Protocols
This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behav
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Enrico Lugli Editor
T-Cell Differentiation Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
T-Cell Differentiation Methods and Protocols
Edited by
Enrico Lugli Laboratory of Translational Immunology, Humanitas Clinical & Research Center, Rozzano, Italy
Editor Enrico Lugli Laboratory of Translational Immunology Humanitas Clinical & Research Center Rozzano, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6546-5 ISBN 978-1-4939-6548-9 (eBook) DOI 10.1007/978-1-4939-6548-9 Library of Congress Control Number: 2016952630 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface T cells are important effectors of the adaptive immune system. Potent T cell responses with superior quality can mediate cancer regression or control chronic viral infections while, on the other hand, exacerbated immune responses may lead to the development of autoimmune diseases. Hence, T cells are not equal in different pathological conditions; rather the T cell compartment is characterized by extreme heterogeneity, with dozens of subsets displaying differential cytokine production, metabolic activity, tissue tropism, immune reconstitution potential, or persistence in the long term. Characterization of context-specific heterogeneity is fundamental to understand the biological basis of disease pathogenesis or to exploit T cell-based responses in immunotherapeutic approaches. This volume reports the description of established and cutting-edge technologies that are helpful to characterize the
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