Ultrasensitive split-type electrochemical sensing platform for sensitive determination of organophosphorus pesticides ba
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RESEARCH PAPER
Ultrasensitive split-type electrochemical sensing platform for sensitive determination of organophosphorus pesticides based on MnO2 nanoflower-electron mediator as a signal transduction system Yuanfang Sun 1 & Pengyuan Xiong 1 & Juan Tang 1 & Zhiyao Zeng 1 & Dianping Tang 2 Received: 22 June 2020 / Revised: 9 July 2020 / Accepted: 15 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Organophosphorus pesticides (OPs) are extensively used worldwide as agrochemicals; however, excess use may threaten the health of humans. Thus, it is an urgent need to develop a sensitive method for determination of OPs. Herein, a simple and sensitive split-type electrochemical method was developed by using MnO2 nanoflower-electron mediator as a signal transduction element. The MnO2 nanoflower-electron mediator was synthesized and shows an excellent electrochemical signal attributed to the high specific surface area of MnO2 nanoflower. Meanwhile, the inhibition of OPs on butyrylcholinesterase (BChE) was carried out in the homogeneous system. In the absence of target molecule, a large number of thiocholines (TCh) were yielded from hydrolysis of acetylthiocholine (ATCh) by BChE. The MnO2 nanoflower was cracked, and subsequently, multiple electron mediator molecules were released from the platform after treated with TCh, thus decreasing the electrochemical response. Furthermore, the inhibition of OPs on BChE resulted in the reduced generation of TCh, thus inducing the recovery of electrochemical signal. Under the optimal experimental, dichlorvos can be detected in a wide range of 10−6–10−10 M, with a detection limit of 3 × 10−10 M. Moreover, the assay was successfully used to analyze dichlorvos in cucumber juice and pear juice, showing a great promising potential for detecting organophosphorus pesticides in complex samples. Keywords Organophosphorus pesticides . MnO2 nanoflower . Split type . Electrochemical method
Introduction The overuse of organophosphorus pesticides (OPs) has aroused serious problems in food safety and human health [1, 2]. OPs can inhibit the acetylcholinesterase activity even at low concentrations by the accumulation of acetylcholine in humans, which induced over-stimulated receptor in synapses and thus cause many diseases [2]. Therefore, it is urgent to Yuanfang Sun and Pengyuan Xiong contributed equally to this work. * Juan Tang [email protected]; [email protected] 1
Department of Chemistry and Chemical Engineering, Ministry of Education Key Laboratory of Functional Small Organic Molecule, Jiangxi Normal University, Nanchang 330022, Jiangxi, China
2
Department of Chemistry, Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education of China and Fujian Province), Fuzhou University, Fuzhou 350108, Fujian, China
design high efficiency methods for tracing of organophosphorus pesticide residues in foods. Classical analytical techniques such as mass spectrometry and liquid chromatography offer an excellent performance [3–5]. However, the high cost o
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