Western Blotting Methods and Protocols
This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular
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Biji T. Kurien R. Hal Scofield Editors
Western Blotting Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Western Blotting Methods and Protocols
Edited by
Biji T. Kurien Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA; Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA
R. Hal Scofield Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA; Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA
Editors Biji T. Kurien Arthritis and Clinical Immunology Oklahoma Medical Research Foundation Oklahoma City, OK, USA
R. Hal Scofield Arthritis and Clinical Immunology Oklahoma Medical Research Foundation Oklahoma City, OK, USA
Department of Medicine, University of Oklahoma Health Sciences Center Oklahoma City, OK, USA
Department of Medicine, University of Oklahoma Health Sciences Center Oklahoma City, OK, USA
Department of Veterans Affairs Medical Center Oklahoma City, OK, USA
Department of Veterans Affairs Medical Center Oklahoma City, OK, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2693-0 ISBN 978-1-4939-2694-7 (eBook) DOI 10.1007/978-1-4939-2694-7 Library of Congress Control Number: 2015938704 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Coomassie stained urea gel and corresponding amido black stained blot of two-dimensional separations of chicken liver ribosomal proteins. Rearranged and colorized photographs
