3D Sponge-Matrix Histoculture Methods and Protocols
This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapte
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Robert M. Hoffman Editor
3D SpongeMatrix Histoculture Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes http://www.springer.com/series/7651
3D Sponge-Matrix Histoculture Methods and Protocols Edited by
Robert M. Hoffman AntiCancer, Inc., San Diego, CA, USA and Department of Surgery, UCSD, San Diego, CA, USA
Editor Robert M. Hoffman AntiCancer, Inc. San Diego, CA, USA Department of Surgery UCSD San Diego, CA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7743-7 ISBN 978-1-4939-7745-1 (eBook) https://doi.org/10.1007/978-1-4939-7745-1 Library of Congress Control Number: 2018935918 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Frontispiece
In Memoriam: Joseph Leighton 1921–1999 Father of 3-Dimensional Tissue Culture
v
This book is dedicated to the memory of A.R. Moossa, M.D., and Sun Lee, M.D.
Preface The Dawn of Tissue Culture Maintaining and growing cells and tissues outside of the body was a momentous achievement that began in the late nineteenth century [1]. Many scientists at that time did not believe that growing cells and tissues outside of the body would be possible. Roux maintained the medullary plate of an embryonic chicken in a saline solution for several days, thereby establishing the principle that tissues could live outside body [1]. In 1907, Harrison [2] observed nerve fibers growing from the explants from the nerve fiber tips of the ta
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