Au Nanoparticle-decorated Nanoporous PEDOT Modified Glassy Carbon Electrode: A New Electrochemical Sensing Platform for
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pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
Au Nanoparticle-decorated Nanoporous PEDOT Modified Glassy Carbon Electrode: A New Electrochemical Sensing Platform for the Detection of Glutathione Rajendran Rajaram, Pandiyaraj Kanagavalli, Shanmugam Senthilkumar, and Jayaraman Mathiyarasu
Recevied: 1 March 2020 / Revised: 8 May 2020 / Accepted: 27 May 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract Glutathione (GSH) is an important bio-thiol which is playing vital role in the human system, such as treating glaucoma, preventing asthma, cancer, etc. Therefore, electrochemical sensing of GSH is becoming very vital to know the level of the biomolecule in living systems. Hence, the present work aims to develop a sensor matrix for electrochemical biosensing of GSH at Au nanoparticle decorated Nanoporous poly(3,4)ethylene dioxythiophene (PEDOT) modified glassy carbon electrode (AuNPPEDOT/GCE). The electrode is modified with a nanoporous fibrillar network of PEDOT containing Au nanoparticles and characterized using UV-Visible spectroscopy and Field Emission Scanning Electron Microscopy (FESEM). The platform is capable of producing enhanced signal against the bio-analyte at the applied overpotential of 0.6 V. Whereas bare glassy carbon electrode is unable to produce the signal. Conversely, Nanoporous PEDOT modified glassy carbon electrode can sense GSH of very low sensitivity at an applied overpotential of 0.7 V. From single step chronoamperometric measurements, kinetic parameters such as diffusion coefficient and apparent rate constant of the reaction were found as 5.01 × 10-5 cm2·s-1 and 1.106 × 103 M-1·s-1 respectively. From the amperometric response, the sensitivity and limit of detection (LOD) of the electrode were to be 10.7 µA cm-2/µM and 0.173 µM respectively with the linear range of 0.5 to 10 µM. The matrix can Rajendran Rajaram*, Jayaraman Mathiyarasu Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Electrochemical Research Institute (CECRI) campus, Chennai-600113, India Tel: +91-4565-241340; Fax: +91-4565-227779 E-mail: [email protected] Rajendran Rajaram, Pandiyaraj Kanagavalli, Shanmugam Senthilkumar, Jayaraman Mathiyarasu Electrodics and Electrocatalysis Division, CSIR-CECRI, Karaikudi-630 003, Tamil Nadu, India
detect GSH in the presence of other possible interfering molecules present in blood samples. Keywords: AuNP-PEDOT/GCE, glutathione, electrochemical (bio)sensing, applied overpotential, limit of detection
1. Introduction Glutathione (GSH) is a tripeptide molecule made up of three amino acids, glutamine, cysteine, and glycine. It is present in our system at the concentration level of 0.1 mM to 1 mM. It is readily oxidized into its disulfide (GSSG). The molecule has vital applications, including the prevention of cells from reactive oxygen intermediates, UV radiation, and toxicity due to heavy metals in the human system [1,2]. GSH concentration of an individual can also be correlated with some biological disorders such as Alzheimer
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