Amperometric determination of Myo-inositol using a glassy carbon electrode modified with nanostructured copper sulfide
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ORIGINAL PAPER
Amperometric determination of Myo-inositol using a glassy carbon electrode modified with nanostructured copper sulfide Rajendran Rajaram 1,2 & Muniyandi Kiruba 3 & Chinnathambi Suresh 2 Shanmugam Kumaran 4 & Ramanathan Kumaresan 5
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Jayaraman Mathiyarasu 1,2
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Received: 15 November 2019 / Accepted: 23 April 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A method for the amperometric determination of Myo-inositol is presented. Nanostructured copper sulfide material was synthesized by solvothermal method and utilized as sensor matrix. The physico-chemical analysis using XRD, Raman, FE-SEM, TEM, and XPS confirmed the formation of CuS material. The voltammetric response of CuS-modified glassy carbon electrode for a successive Myo-inositol (0.5 μM) addition confirmed that the reaction takes place at the surface of the electrode. The modified electrode resulted in signal enhancement for a linear response ranging from 0.5–8.5 μM at an applied overpotential of 0.65 V with a correlation coefficient value (R2) of 0.99. The sensitivity and limit of detection of the modified electrode were 7.87 μA μM−1 cm−2 and 0.24 μM, respectively. The interfering effect of various compounds present in real samples was examined. Keywords Electrochemical sensor . Diabetes mellitus . Urine analysis . Solvothermal synthesis . Modified electrode
Introduction Since diabetes mellitus (DM) is a major health concern, determination of glucose level in an every individual has gained enormous attention in the fields of research and health care [1–5]. Indirect estimation of glucose in DM patient needs tiny amount of blood sample which is obtained by a painful prick. In an alternate way, recently, it is reported that the onset of DM can be diagnosed at the early stage using urinary Myo-inositol (MI) as one of the biomarkers using the enzyme, MI
dehydrogenase (MIDH) [6]. MI is a stereoisomer of inositol, which is a cyclic isomer of D-glucose. In glucose metabolism, insulin on binding to its receptor activates the phospholipaseC which in turn hydrolyzes phosphatidylinositol into inositol and diacylglycerol (DAG). During this metabolism, glucose and MI are reabsorbed by the renal tubules and only around 1– 2% of MI is excreted via urine [7]. However, when the blood glucose level increases, the renal tubules absorb glucose and excrete MI via urine thereby increasing the levels of MI in the urine [8]. Measurement of urinary MI (ΔUMI) after glucose
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04300-z) contains supplementary material, which is available to authorized users. * Chinnathambi Suresh [email protected] * Jayaraman Mathiyarasu [email protected] 1
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Academy of Scientific and Innovative Research (AcSIR), CSIR Central Electrochemical Research Institute (CECRI) Campus, Chennai 600113, India Electrodics and Electrocatalysis Division, CSIR- Central Electrochemical Research Institute, Karaikudi, Tamil Nadu 630 003, India
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PG & Research D
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