Luteolibacter luteus sp. nov., isolated from stream bank soil
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ORIGINAL PAPER
Luteolibacter luteus sp. nov., isolated from stream bank soil Ram Hari Dahal1 · Dhiraj Kumar Chaudhary2 · Dong‑Uk Kim3 · Jaisoo Kim1 Received: 27 July 2020 / Revised: 7 September 2020 / Accepted: 12 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A non-motile, Gram-stain-negative, rod-shaped and yellow-colored bacterium, designated G-1-1-1T was obtained from soil sampled at Gwanggyo stream bank, Gyeonggi-do, Republic of Korea. Cells were aerobic, catalase positive, grew optimally at 25–30 °C and hydrolysed aesculin and casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-1-1-1T formed a lineage within the genus Luteolibacter. The closest members were Luteolibacter flavescens GKXT (97.7% sequence similarity) and Luteolibacter arcticus MC 3 726T (97.3%). The sequence similarities with other members of the genus Luteolibacter were ≤ 93.9%. The genome of strain G-1-1-1T was 6,412,079 bp long with 5176 protein-coding genes. The diagnostic amino acid of cell-wall peptidoglycan of strain G-1-1-1T was meso-diaminopimelic acid. The only respiratory quinone was menaquinone-9 and the principal polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. The predominant cellular fatty acids were iso-C 14:0, C16:1 ω9c, C16:0, C14:0 and anteiso-C15:0. The DNA G + C content was 61.0 mol%. The anti-SMASH analysis of whole genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain G-1-1-1T represents a novel species in the genus Luteobacter, for which the name Luteolibacter luteus sp. nov. is proposed. The type strain is G-1-1-1T (= KACC 21614T = NBRC 114341T). Keywords Luteolibacter luteus · Verrucomicrobia · Verrucomicrobiaceae · Stream bank soil · Meso-diaminopimelic acid
Introduction The genus Luteolibacter was first proposed by Yoon et al. with the type species Luteolibacter pohnpeiensis in the family Verrucomicrobiaceae within the phylum Verrucomicrobia (Yoon et al. 2008). The genus has been subsequently emended by Jiang et al. (Jiang et al. 2012) and Kim et al. Communicated by Erko stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02048-x) contains supplementary material, which is available to authorized users. * Jaisoo Kim [email protected] 1
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi‑Do 16227, Republic of Korea
2
Department of Environmental Engineering, Korea University Sejong Campus, Sejong City 30019, Republic of Korea
3
Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea
(Kim et al. 2015). To date, there are eight species of the genus Luteolibacter with validly published names (https:// lpsn.dsmz.de/genus/luteolibacter). Representatives of
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