Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western H
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ORIGINAL PAPER
Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western Himalaya Rishabh Kaushik . Meesha Sharma . Kumar Gaurav . U. Jagadeeshwari . A. Shabbir . Ch. Sasikala . Ch. V. Ramana . Maharaj K. Pandit
Received: 8 June 2020 / Accepted: 1 September 2020 Ó Springer Nature Switzerland AG 2020
Abstract A novel strain of Planctomycetes, designated JC670T, was isolated from a high altitude (* 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding. Strain JC670T was found to grow well at pH 7.0 and pH 8.0 and to tolerate up to 2% NaCl (w/v). MK6 was the only
Rishabh Kaushik and Meesha Sharma having equal authorship and consider as first authors.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01471-w) contains supplementary material, which is available to authorized users. R. Kaushik M. Sharma M. K. Pandit (&) Department of Environmental Studies, University of Delhi, Delhi 110007, India e-mail: [email protected] K. Gaurav A. Shabbir Ch. V. Ramana (&) Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India e-mail: [email protected] U. Jagadeeshwari Ch. Sasikala Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500085, India
respiratory quinone identified. The major fatty acids of strain JC670T were identified as C18:1x9c, C18:0 and C16:0, and phosphatidylcholine, two unidentified phospholipids and six unidentified lipids are present as the polar lipids. The polyamines putrescine and sym-homospermidine were detected. Strain JC670T shows high 16S rRNA gene sequence identity (95.4%) with Paludisphaera borealis PX4T. The draft genome size of strain JC670T is 7.97 Mb, with G ? C content of 70.4 mol%. Based on phylogenetic analyses with the sequences of ninety-two core genes, low dDDH value (20.6%), low gANI (76.8%) and low AAI (69.1%) results, differential chemotaxonomic and physiological properties, strain JC670T (= KCTC 72850T = NBRC 114339T) is recognised as the type strain of a new species of the genus Paludisphaera, for which we propose the name Paludisphaera soli sp. nov. Keywords Soil bacteria Western himalaya Planctomycetes Isosphaeraceae Paludisphaera High altitude Abbreviations NCBI National Center for Biotechnology Information ANI Average Nucleotide Index AAI Average Nucleotide Index dDDH Digital DNA-DNA Hybridization HPLC High-Pressure Liquid Chromatography KCTC Korean Collection for Type Cultures
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Antonie van Leeuwenhoek
Introduction The phylum Planctomycetes is an important member of the Planctomycetes/Verrucomicrob
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