Drosophila Methods and Protocols
This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols, and current methods that cover recent breakthroughs in Drosophila
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Christian Dahmann Editor
Drosophila Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Drosophila Methods and Protocols Second Edition
Edited by
Christian Dahmann Institute of Genetics, Technische Universität Dresden, Dresden, Germany
Editor Christian Dahmann Institute of Genetics Technische Universität Dresden Dresden, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6369-0 ISBN 978-1-4939-6371-3 (eBook) DOI 10.1007/978-1-4939-6371-3 Library of Congress Control Number: 2016948818 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface The fruit fly Drosophila melanogaster is one of the most powerful genetically tractable model organisms. Work using Drosophila has made many valuable contributions to our understanding of animal development, behavior, and physiology and of human disease. Inspiring work and new research avenues often depend on the development of novel techniques. The use of transposons to generate transgenic flies and the adaptation of yeast systems to express genes or make mosaic animals are to name but a few of the techniques that led to breakthroughs in Drosophila research. Descriptions of these and other important methods were included in the first edition of Drosophila: Methods and Protocols published in 2008. Since then, the thriving fly researcher community continued to refine and invent techniques. The current second edition of this book in part tries to reflect this effort and covers some of these more recent methods. The book starts with a description of FlyBase, a database of genes and gen
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