Flavobacterium ajazii sp. nov., Isolated from Seaweed of Gouqi Island, China
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Flavobacterium ajazii sp. nov., Isolated from Seaweed of Gouqi Island, China Ishrat Khan1 · Sanjit Chandra Debnath1 · Cen Yan1 · Can Chen1 · Yan Xu1 · Wen‑Jie Wang1 · Yu‑Chao Yu1 · Dao‑Qiong Zheng1 · Jin‑Zhong Xu1 · Pin‑Mei Wang1 Received: 27 February 2020 / Accepted: 30 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel bacterium designated SSM4.2T was isolated from seaweed of Gouqi Island, which is the center of the Zhoushan fishing ground in the East China Sea. Strain SSM4.2T was Gram-stain-negative, bright yellow-pigmented, short rod-shaped, non-flagellated, non-spore forming, aerobic and motile by gliding. Growth was observed at 4–37 °C (optimum 25–30 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–2.0% (w/v) NaCl (optimum 0%) concentration. The strain was catalase- and oxidasepositive. Menaquinone-6 (MK-6) was found as the sole respiratory quinone and zeaxanthin as the main carotenoid pigment. The predominant fatty acids (≥ 10%) were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c /C16:1 ω6c). The major polar lipid was phosphatidylethanolamine (PE). The genome size was 5.7 Mbp. The DNA G + C content was 34.1 mol%. 16S rRNA gene sequence revealed that strain SSM4.2T belongs to the genus Flavobacterium and shares high-sequence similarity with F. limi KACC 1 8851T (98.1%), F. hydrophilum KACC 1 9591T (97.6%), F. defluvii KCTC 12612T (97.1%), F. cheongpyeongense KACC 1 9592T (97.0%) and F. fluviatile KCTC 5 2446T (96.9%). Strain SSM4.2T had 73.2–84.6% average nucleotide identity and 19.1–29.4% digital DNA-DNA hybridization values with its closest type strains. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic features, strain SSM4.2T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ajazii sp. nov. is proposed. The type strain is SSM4.2T (= KCTC 72807T = MCCC 1K04370T).
Introduction The genus Flavobacterium (belongs to the family Flavobacteriaceae) was first described by Bergey et al. [1]. Up to July 2020, this genus constitutes 229 species that are validly published with valid names (LPSN; https://lpsn.dsmz. de/genus/flavobacterium). Members of the genus Flavobacterium are reported from diverse habitats, including fresh water [2], sea water [3], forest soil [4], stream bank soil Ishrat Khan and Sanjit Chandra Debnath contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02112-1) contains supplementary material, which is available to authorized users. * Pin‑Mei Wang [email protected] 1
[5], rhizosphere of wetland [6], wastewater treatment plant [7], oil-contaminated arctic soil [8] and glacier [9]. During our study to explore marine microbial resources in the East China Sea, a novel Flavobacterium species designated as SSM4.2T was isolated from seaweed of Gouqi Island. Gouqi Island is located in the east of the East China Sea. It carries a very rich ecosystem [10]. Gouqi Island possesses ab
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