Flavobacterium agri sp. nov., a novel bacterial species isolated from rhizospheric soil of Coriandrum sativum
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ORIGINAL PAPER
Flavobacterium agri sp. nov., a novel bacterial species isolated from rhizospheric soil of Coriandrum sativum Shahina Akter1 · Sun‑Young Lee2 · BoKyung Moon2 · Md. Amdadul Huq2 Received: 18 June 2020 / Revised: 14 September 2020 / Accepted: 30 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain-negative, strictly aerobic, cream yellow colored, non-motile, rod-shaped bacterial strain, designated MAH-1 T was isolated from rhizospheric soil of Coriandrum sativum. A polyphasic taxonomic study was performed on the isolated strain. Optimal growth occurred at 28–30 °C, pH 6.5 and 0% NaCl. The strain showed activity for both catalase and oxidase tests. Cell growth occurs on R2A agar, nutrient agar and Luria–Bertani agar. Cells were able to hydrolyze starch, aesculin, gelatin, and Tween 20. Alignment of 16S rRNA gene sequences indicated that strain MAH-1T was associated with the genus Flavobacterium and was most closely related to Flavobacterium longum YIT 12745T (94.5% sequence similarity) and Flavobacterium caeni LM5T (93.0%). Strain MAH-1T had a genome size of 3,975,600 bp. Genome contained 67 contigs encoded by 3,522 protein-coding genes with 38 tRNA and 6 rRNA genes. The genomic DNA G + C contents of strain MAH-1T was 47.1 mol %. The genomic ANI and dDDH values between strain MAH-1T and one of the close relatives F. caeni LM5T were 72.2 and 18.8%, respectively. The major fatty acids were C15:0 iso, C16:0 iso and C15:0 anteiso. The predominant respiratory quinone was menaquinone 6 (MK-6). Based on physiological, biochemical and phylogenetic data for this isolate, it was confirmed that strain MAH-1T was affiliated to the genus Flavobacterium and represented a novel species, for which the name Flavobacterium agri sp. nov. is proposed. The type strain is MAH-1T (= KACC 19300T = CGMCC 1.16617T). Keywords Flavobacterium agri · Gram-staining negative · Menaquinone 6 · Genome sequence
Introduction The genus Flavobacterium, type genus of the family Flavobacteriaceae (Ludwig et al. 2011), was established by Bergey et al. (1923) and emended by Bernardet et al. (1996), Communicated by Erko stackebrandt. The NCBI GenBank accession numbers for the 16S rRNA gene and draft-genome sequence of strain MAH-1T are KY964281 and JABWMI000000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02068-7) contains supplementary material, which is available to authorized users.
Kuo et al. (2013), Kang et al. (2013) and Dong et al. (2013). Members of the genus Flavobacterium were isolated from different habitats such as plant, animal, algae, seawater, freshwater, soil, compost, sediment, lagoon, Antarctic lakes, glacier, activated sludge, water reservoir and wastewater treatment plant sources (LPSN; www.bacter io.net/flavo bacterium.html). During investigations on the biodiversity of bacteria in the soil of coriander garden, a novel bacterium, designated strain MAH-1T, was isolated. The current stu
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