Protocols for Neural Cell Culture Fourth Edition
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth editi
- PDF / 8,030,479 Bytes
- 395 Pages / 547.087 x 737.008 pts Page_size
- 33 Downloads / 214 Views
For other titles published in this series, go to www.springer.com/series/8623
Protocols for Neural Cell Culture Edited by
Laurie C. Doering, PhD McMaster University, Hamilton, ON, Canada
Fourth Edition
Editor Laurie C. Doering Department of Pathology & Molecular Medicine McMaster University 1200 Main Street West Health Sciences Centre Hamilton ON L8N 3Z5 Canada [email protected]
ISBN 978-1-60761-291-9 e-ISBN 978-1-60761-292-6 DOI 10.1007/978-1-60761-292-6 Library of Congress Control Number: 2009932368 © Humana Press, a part of Springer Science+Business Media, LLC 1992, 1997, 2001, 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper springer.com
Preface Ross G. Harrison’s founding experiments with nerve cell cultures between 1907 and 1910 ´ y Cajal centered on the “Neuwere motivated by the same concerns of Santiago Ramon ron Doctrine.” Cajal used impressive silver impregnations in chick spinal cord to illustrate axonal growth in vivo. Harrison, using pieces of frog neural tube nourished in lymph within a depression slide, solved the problems of his predecessors and devised a reproducible method of tissue culture experimentation. For the first time, the outgrowth rates of individual fibers and their growth cones were observed in real time under the microscope. In essence, Harrison established a direct in vitro test to prove Cajal’s axon outgrowth theory. Harrison overcame basic tissue culture problems and created a culture technique others could follow. Since the beginning of nervous system tissue culture with Ross Harrison’s vision, now just over 100 years ago, the scientific community has established numerous protocols to generate the current wide variety of cell and tissue culture technology. The rapid growth of neuroscience during the 1980s and a highly acclaimed, intensive tissue culture course held in Saskatoon, Saskatchewan, for several years formed the concept for this protocol series in neural tissue culture. In 1992, Dr. Sergey Fedoroff (Un
Data Loading...
