Identification of recombination in novel goose parvovirus isolated from domesticated Jing-Xi partridge ducks in South Ch
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ORIGINAL PAPER
Identification of recombination in novel goose parvovirus isolated from domesticated Jing‑Xi partridge ducks in South China Wen‑jun Liu1,2 · You‑tian Yang3 · Hai‑yin Zou1 · Shi‑jian Chen1,2 · Chen Yang1,2 · Yun‑bo Tian1,2 · Yun‑mao Huang1,2 Received: 23 March 2020 / Accepted: 7 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Outbreaks of short beak and dwarfism syndrome (SBDS), caused by a novel goose parvovirus (NGPV), have occurred in China since 2015. This rapidly spreading, infectious disease affects ducks in particular, with a high morbidity and low mortality rate, causing huge economic losses. This study analyzed the evolution of NGPV isolated from Jing-Xi partridge duck with SBDS in South China. Complete genome sequences of the NGPV strains GDQY1802 and GDSG1901 were homologous with other GPV/NGPV and Muscovy duck parvovirus (MDPV) strains. Phylogenetic analysis showed that the NGPV isolated from mainland China was related to the Taiwan 82-0321v strain of GPV. In contrast to 82-0321v and the SDLC01 strain, which was first isolated from China, the two isolates showed no deletions in the inverted terminal repeat (ITR) region. Further, in these isolates, 24 amino acid sites of the replication protein were different compared to that of GPV live vaccine strain 82-0321v, and 12 sites were unique across all NGPV isolates. These isolates also showed differences in 17 amino acid sites of the capsid protein from that of 82-0321v, two of which were the same as those in MDPV. Recombination analysis identified the major parents of GDSG1901 and GDQY1802 as the NGPV-GD and NGPV-Hun18 strains, and the minor parents as the classical GPV 06-0329 and GPV LH strains, respectively. GDQY1802 and GDSG1901 are recombinant GPVrelated parvovirus isolated from domesticated partridge duck. Recombination is evident in the evolution of NGPV, and as such, the use of live attenuated vaccines for NGPV requires further study. Keywords Partridge duck · Novel goose parvovirus · Phylogenetic · Recombination
Introduction Viruses of the genus Dependovirsu, which includes goose parvovirus (GPV), novel goose parvovirus (NGPV) and Muscovy duck parvovirus (MDPV), belong to the family Edited by William Dundon. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11262-020-01781-1) contains supplementary material, which is available to authorized users. * Yun‑bo Tian [email protected] * Yun‑mao Huang [email protected] 1
Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
2
Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, Guangdong, China
3
Foshan University, Foshan 528000, Guangdong, China
Parvoviridae, and are important pathogens of waterfowl [1]. The host ranges and pathogenicities of GPV, NGPV and MDPV differ, although they have high gene homology. The host range of MDPV is very narrow. It occurs mainly in three-week-old Muscovy ducklings, is characterized by loc
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