Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil
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ORIGINAL PAPER
Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil Shahina Akter1 · Jae Hee Park2 · Chang Shik Yin3
Received: 8 September 2015 / Revised: 11 December 2015 / Accepted: 23 December 2015 © Springer-Verlag Berlin Heidelberg 2016
Abstract A novel bacterial strain THG-SL1T was isolated from a soil sample of Cucurbita maxima garden and was characterized by using a polyphasic approach. Cells were Gram-reaction-positive, non-motile and rod-shaped. The strain was aerobic, catalase positive and weakly positive for oxidase. Phylogenetic analysis based on 16S rRNA gene sequence analysis but it shared highest similarity with Microbacterium ginsengisoli KCTC 19189T (96.6 %), indicating that strain THG-SL1T belongs to the genus Microbacterium. The DNA G + C content of the isolate was 68.9 mol %. The major fatty acids were anteisoC15: 0 (39.7 %), anteiso-C17: 0 (24.4 %) and iso-C16: 0 (18.5 %). The major polar lipids of strain THG-SL1T were Communicated by Erko Stackebrandt. Shahina Akter and Jae Hee Park have equally contributed to this work. The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-SL1T is KM576855. Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1183-3) contains supplementary material, which is available to authorized users. * Chang Shik Yin [email protected] 1
Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446‑701, Republic of Korea
2
Graduate School of Biotechnology, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 446‑701, Republic of Korea
3
Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, # 1 Hoegi‑dong, Dongdaemun‑gu, Seoul 130‑701, Republic of Korea
phosphatidylglycerol (PG) and an unidentified glycolipid (GL). The predominant respiratory isoprenoid quinones were menaquinone-11 and menaquinone-12. The diamino acid in the cell-wall peptidoglycan was ornithine. Based on the results of polyphasic characterization, strain THGSL1T represented a novel species within the genus Microbacterium, for which the name Microbacterium horti sp. nov. is proposed. The type strain is THG-SL1T (=KACC 18286T=CCTCC AB 2015117T). Keywords Microbacterium horti · Gram-reactionpositive · Polyphasic taxonomy · 16S rRNA
Introduction The genus Microbacterium, belonging to the family Microbacteriaceae, phylum Actinobacteria, was first described by Orla-Jensen (1919) and emended by Collins et al. (1983) and by Takeuchi and Hatano (1998). Members of the genus Microbacterium are short rods, which are generally aerobic and catalase positive (Collins and Bradbury 1992). The major polar lipids are diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and unidentified glycolipids (GL), and the G + C content of the DNA ranges from 61 to 75 % (Alves et al. 2014, Takeuchi and Hatano 1998). Members of the genus Microbacterium play different role
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