Sphingomonas horti sp. nov., a novel bacterial species isolated from soil of a tomato garden
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ORIGINAL PAPER
Sphingomonas horti sp. nov., a novel bacterial species isolated from soil of a tomato garden Shahina Akter1 · Sun‑Young Lee2 · Sung‑Kwon Moon2 · Changsun Choi2 · Sri Renukadevi Balusamy3 · Muhammad Zubair Siddiqi4 · Md. Ashrafudoulla5 · Md. Amdadul Huq2 Received: 30 January 2020 / Revised: 10 May 2020 / Accepted: 17 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel bacterial strain, designated MAH-20T, was isolated from a soil sample of a tomato garden. Cells of strain MAH20T were Gram-stain negative, aerobic, motile, and rod-shaped. The colonies were light brown colored, smooth, spherical, and 0.2–0.7 mm in diameter when grown on Luria–Bertani agar for 2 days. Strain MAH-20T grows at 15–40 °C (optimum growth temperature 30–32 °C), at pH 5.0–10.0 (optimum growth pH 7.0) and at 0–2.0% NaCl. The strain showed positive activity for both oxidase and catalase tests. Cells were able to hydrolyze starch, DNA, urea, gelatin, L-arginine, and Tween 20. According to the 16S rRNA gene sequence similarity, the strain MAH-20T was identified as a new member of the genus Sphingomonas and had the close sequence similarity with Sphingomonas changbaiensis V2M44T (98.9%) and Sphingomonas tabacisoli X1-8T (98.1%). The genomic ANI value between strain MAH-20T and S. changbaiensis NBRC 104936T was 84.4%. The novel strain MAH-20T has a draft genome size of 3,350,026 bp (25 contigs), annotated with 3210 protein-coding genes, 46 tRNA, and 3 rRNA genes. The genomic DNA G + C content of isolate was 67.3 mol%, the predominant quinone was ubiquinone 10 and the major fatty acids were C16:0, C17:1 ω6c and summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c). On the basis of DNA-DNA hybridization results, phenotypic, genotypic, and chemotaxonomic data, the isolated strain MAH-20T represents a novel species, for which the name Sphingomonas horti sp. nov. is proposed, with MAH-20T as the type strain (= KACC 19746T = CGMCC1.13658T). Keywords Sphingomonas horti · Gram-staining negative · Genome sequence · 16S rRNA
Introduction
Communicated by Erko Stackebrandt. The NCBI GenBank accession number for the 16S rRNA gene and draft genome sequences of strains MAH-20T is MH368767 and WQMS00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02056-x) contains supplementary material, which is available to authorized users. * Md. Amdadul Huq [email protected]; [email protected] 1
Department of Food Science and Biotechnology, Gachon University, Songnam 461‑701, Republic of Korea
Department of Food and Nutrition, Chung-Ang University, Anseong, Gyeonggi‑do 17546, Republic of Korea
2
The genus Sphingomonas of the family Sphingomonadaceae, belonging to the phylum Proteobacteria, was first described by Yabuuchi et al. (1990). Species of the genus Sphingomonas were isolated from various sources, including soil, water, air, desert, sediment, hospital respirator, plant roots, plant tissues, rice paddy, a
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