Paucibacter aquatile sp. nov. isolated from freshwater of the Nakdong River, Republic of Korea
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ORIGINAL PAPER
Paucibacter aquatile sp. nov. isolated from freshwater of the Nakdong River, Republic of Korea Young Ho Nam1 · Ahyoung Choi1 · Jung Moon Hwang1 · Kyung June Yim1 · Jee‑Hwan Kim1 · Gang‑Guk Choi1 · Eu Jin Chung1 Received: 23 November 2017 / Revised: 18 January 2018 / Accepted: 9 February 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract A Gram-negative, aerobic, motile, and rod-shaped bacterial strain designated C R182T was isolated from freshwater of the Nakdong River, Republic of Korea. Optimal growth conditions for this novel strain were found to be: 25–30 °C, pH 6.5–8.5, and 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicates that the strain CR182T belongs to type strains of genus Paucibacter. Strain CR182T showed 98.0% 16S rRNA gene sequence similarity with Paucibacter oligotrophus CHU3T and formed a robust phylogenetic clade with this species. The average nucleotide identity value between strain CR182T and P. oligotrophus CHU3T was 78.4% and the genome-to-genome distance was 22.2% on average. The genomic DNA G+C content calculated from the genome sequence was 66.3 mol%. Predominant cellular fatty acids of strain C R182T were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) (31.2%) and C16:0 (16.0%). Its major respiratory quinine was ubiquinone Q-8. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified phospholipids. Its genomic DNA G+C content was 66.3%. Based on data obtained from this polyphasic taxonomic study, strain C R182T represents a novel species belonging to genus Paucibacter, for which a name of P. aquatile sp. nov. is proposed. The type strain is CR182T (= KCCM 90284T = NBRC 113032T). Keywords Paucibacter aquatile · Novel species · Polyphasic taxonomy
Introduction The genus Paucibacter was first proposed by Rapala et al. (2005) with Paucibacter toxinivorans as type species belonging to family Comamonadaceae in phylum Betaproteobacteria. This strain was able to degrade microcystins and nodularin produced by cyanobacteria in aquatic
Communicated by Erko Stackebrandt. Young Ho Nam and Ahyoung Choi equally contributed. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-018-1494-2) contains supplementary material, which is available to authorized users. * Eu Jin Chung [email protected] 1
Freshwater Bioresources Culture Research Bureau, Nakdonggang National Institute of Biological Resources (NNIBR), 137, Donam 2‑gil, Sangju‑si, Gyeongsangbuk‑do 37242, Republic of Korea
environments (Rapala et al. 2005). At the time of writing, this genus consists of two described species isolated from freshwater environments: P. toxinivorans and P. oligotrophus (Pheng et al. 2017). During a screening of bacteria with antimicrobial activity, a novel strain C R182T was isolated from freshwater in the Nakdong River, Korea. Paucibacter species are Gram-negative, aerobic, motile, and rod-shaped bacteria containing ubiquinone Q-8. In t
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