Application of 1 H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots
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ORIGINAL ARTICLE
Application of 1H‑NMR combined with qRT‑PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib Zhuoni Hou1 · Yuanyuan Li1 · Feng Su2 · Jipeng Chen2 · Xiaodan Zhang1 · Ling Xu1 · Dongfeng Yang1 · Zongsuo Liang1 Received: 8 September 2020 / Accepted: 29 October 2020 © The Author(s) 2020
Abstract Main conclusion Methyl jasmonate promotes the synthesis of rosmarinic acid in Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib, and it promotes the latter more strongly. Abstract Salvia miltiorrhiza Bunge (SMB) is a traditional Chinese medicinal material, its water-soluble phenolic acid component rosmarinic acid has very important medicinal value. Salvia castanea f. tomentosa Stib (SCT) mainly distributed in Nyingchi, Tibet. Its pharmacological effects are similar to SMB, but its rosmarinic acid is significantly higher than the former. Methyl jasmonate (MJ) as an inducer can induce the synthesis of phenolic acids in SMB and SCT. However, the role of MJ on rosmarinic acid in SMB is controversial. Therefore, this study used SMB and SCT hair root as an experimental material and MJ as a variable. On one hand, exploring the controversial reports in SMB; on the other hand, comparing the differences in the mechanism of action of MJ on the phenolic acids in SMB and SCT. The content of related metabolites and the expression of key genes in the synthesis pathway of rosmarinic acid was analyzed by 1H-NMR combined with qRT-PCR technology. Our research has reached the following conclusions: first of all, MJ promotes the accumulation of rosmarinic acid and related phenolic acids in the metabolic pathways of SMB and SCT. After MJ treatment, the content of related components and gene expression are increased. Second, compared to SMB, SCT has a stronger response to MJ. It is speculated that the different responses of secondary metabolism-related genes to MJ may lead to different metabolic responses of salvianolic acid between the two. Keywords 1H-NMR · Methyl jasmonate · qRT-PCR · Rosmarinic acid · Salvia castanea Diels f. tomentosa Stib · Salvia miltiorrhiza Bunge
Communicated by Soheil S. Mahmoud. Zhuoni Hou and Yuanyuan Li contributed equally. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03506-y) contains supplementary material, which is available to authorized users. * Zongsuo Liang [email protected] 1
The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Abbreviations C4H Cinnamate 4-hydroxylase 4CL 4-Coumarate: CoA ligase CYP98A14 Cytochrome P450 HPPR 4-Hydroxyphenylpyruvate reductase MJ Methyl jasmonate PAL Phenylalanine ammonia-lyase PAS Rosmarinic acid synthase SCT Salvia castanea f. tomentosa Stib 2
Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, C
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