Astrocytes Methods and Protocols

This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues

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Barbara Di Benedetto Editor

Astrocytes Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Astrocytes Methods and Protocols

Edited by

Barbara Di Benedetto Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany

Editor Barbara Di Benedetto Department of Psychiatry and Psychotherapy University of Regensburg Regensburg, Germany

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9067-2 ISBN 978-1-4939-9068-9 (eBook) https://doi.org/10.1007/978-1-4939-9068-9 Library of Congress Control Number: 2018967308 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: This cover image comes from Figure 5D of Chapter 19, with permission of the author This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Astrocytes are essential players in the guidance of a myriad of functional programs in the central nervous system (CNS). Among others, they influence a proper wiring of neuronal networks, instruct the formation and functions of the blood-brain barrier, regulate neuronal energy metabolism and cellular circadian rhythm, and control neurogenesis and synaptogenesis. To support both experienced and beginner scientists in unraveling novel, fascinating roles of these versatile cells, this book gathers together current overviews and a broad variety of methods essential to investigating the biology of astrocytes and their multifaceted roles in both healthy and diseased brains.