Chromatin Immunoprecipitation Methods and Protocols
This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance
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Neus Visa Antonio Jordán-Pla Editors
Chromatin Immunoprecipitation Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Chromatin Immunoprecipitation Methods and Protocols
Edited by
Neus Visa and Antonio Jordán-Pla Department of Molecular Biosciences, The Wenner-Gren Institute Stockholm University, Stockholm, Sweden
Editors Neus Visa Department of Molecular Biosciences The Wenner-Gren Institute Stockholm University Stockholm, Sweden
Antonio Jorda´n-Pla Department of Molecular Biosciences The Wenner-Gren Institute Stockholm University Stockholm, Sweden
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7379-8 ISBN 978-1-4939-7380-4 (eBook) DOI 10.1007/978-1-4939-7380-4 Library of Congress Control Number: 2017953868 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Chromatin immunoprecipitation (ChIP) is a powerful technique used to detect, map, and quantify interactions between a protein of interest and genomic DNA in vivo. In a typical ChIP experiment, cells or tissues are treated with formaldehyde to generate reversible crosslinks between DNA and other molecules located in the chromatin, typically proteins but also RNAs. The crosslinked chromatin is extracted and fragmented, and the resulting material is immunoprecipitated with antibodies against a protein of interest. The immunoprecipitated DNA is finally purified and analyzed. When ChIP is used for s
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