First report of a novel partitivirus from the phytopathogenic fungus Fusarium cerealis in China
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First report of a novel partitivirus from the phytopathogenic fungus Fusarium cerealis in China Irfan Ahmed1 · Pengfei Li1,2 · Lihang Zhang1 · Xinwei Jiang3 · Pallab Bhattacharjee1 · Lihua Guo1 · Shuangchao Wang1 Received: 19 June 2020 / Accepted: 8 August 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract To our knowledge, no mycoviruses have been reported in Fusarium cerealis. Here, we describe a novel double-stranded RNA (dsRNA) virus, Fusarium cerealis partitivirus 1 (FcPV1), isolated from F. cerealis strain HN30 from Henan Province, China. The FcPV1 genome consists of two dsRNA segments, 1732 bp (dsRNA1) and 1361 bp (dsRNA2) in length, each containing a single open reading frame potentially encoding a 61.0-kDa protein and a 42.0-kDa protein, respectively. dsRNA1 encodes a putative RNA-dependent RNA polymerase (RdRp), while the dsRNA2 product has no significant similarity to any other capsid proteins (CPs) in the GenBank databases other than limited similarity to hypothetical “capsid” proteins of a few partitiviruses. Sequence alignments and phylogenetic analysis showed that FcPV1 is related to members of the newly proposed genus “Zetapartitivirus” in the family Partitiviridae. Mycoviruses are known to infect almost all taxa of fungi, including plant-pathogenic fungal species [6, 16]. Mycoviruses containing double-stranded RNA (dsRNA) genomes are currently classified into six established families (Chrysoviridae, Megabirnaviridae, Partitiviridae, Quadriviridae, Reoviridae, and Totiviridae) and three proposed families (“Alternaviridae”, “Botybirnaviridae”, and “Fusagraviridae”) [6, 15]. The family Partitiviridae includes five genera: Alphapartitivirus, Betapartitivirus, Cryspovirus,
Handling Editor: Ioly Kotta-Loizou. Irfan Ahmed and Pengfei Li contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04802-4) contains supplementary material, which is available to authorized users. * Shuangchao Wang [email protected] 1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
2
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, China
3
School of International Education, Beijing University of Chemical Technology, Beijing, China
Deltapartitivirus, and Gammapartitivirus [13]. Recently, two new genera, “Epsilonpartitivirus” and “Zetapartitivirus”, have been proposed in this family [7, 9]. Partitiviruses have isometric, nonenveloped virions of 25–43 nm in diameter [10]. They have dsRNA genome segments, dsRNA1 and dsRNA2, that are ~1.4–2.4 kb in length [13]. Fusarium cerealis is a ubiquitous pathogen that is responsible for Fusarium head blight on barley [17], ear rot in maize [11], root rot on ginseng and soybean [1, 5] and dry rot of seed potato tubers [4]. F. cerealis
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