Lactobacillus salivarius NK02: a Potent Probiotic for Clinical Application in Mouthwash
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Lactobacillus salivarius NK02: a Potent Probiotic for Clinical Application in Mouthwash Neda Sajedinejad 1,2,3 & Mojgan Paknejad 1 & Behzad Houshmand 4 & Hakimeh Sharafi 2 & Reza Jelodar 3 & Hossein Shahbani Zahiri 2 & Kambiz Akbari Noghabi 2
# Springer Science+Business Media New York 2017
Abstract A specific strain of naturally occurring oral lactobacilli was isolated and identified based on morphological, biochemical, and 16S rRNA gene sequencing. The phylogenetic affiliation of the isolate confirmed that the NK02 strain had close association with the Lactobacillus salivarius. An effective mouthwash was developed for treatment of periodontitis and suppression of the indicator bacterium Aggregatibacter actinomycetemcomitans which is an obvious pathogen of periodontal disease. The mouthwash containing L. salivarius NK02 was tested at a dose level of 108 (colony forming units (CFU) ml−1), monitoring over a period of 4 weeks. The study was a randomized double-blind placebo control trial, and the patients were treated in two groups of control and test by using scaling and root planing (SRP) + placebo and scaling and root planing (SRP) + probiotic, respectively. It appeared that the probiotic mouthwash was able to inhibit the bacterial growth on both saliva and sub-gingival crevice and exhibited antibacterial activity against A. actinomycetemcomitans. The results also showed that SRP+ probiotic treatment led to a significant decrease of
Neda Sajedinejad and Mojgan Paknejad equally contributed. * Kambiz Akbari Noghabi [email protected] 1
Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
2
Division of Environmental & Industrial Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran
3
Faculty of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
4
Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
gingival index (GI) and bleeding on probing (BOP) compared with that of SRP + placebo for the probiotic group. The rate of decrease in pocket depth was displayed in the group with SRP + probiotic treatment equal to 1/2 mm, and probing pocket depth (PPD) value was decreased in the probiotic bacteria treatment group that can explain the decrease in inflammation in gingiva. Our findings suggest that probiotic mouthwash is healthy for daily use as an alternative for maintaining dental and periodontal health. Keywords Probiotic . Lactobacillus salivarius NK02 . Aggregatibacter actinomycetemcomitans . Periodontal diseases
Introduction The use of probiotics in the medical field is growing as more evidence accumulates supporting their benefits. Given that probiotic bacteria impact human health, applying probiotics to prevent or treat a wide range of diseases has appeared to be a promising new therapy [1–3]. However, the act of choosing microorganisms for use as probiotic treatments should follow careful consideration procedure. Application of probiotics for oral health care has received much gre
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