Lentibacillus saliphilus . sp. nov., a moderately halophilic bacterium isolated from a saltern in Korea
- PDF / 906,925 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 71 Downloads / 234 Views
ORIGINAL PAPER
Lentibacillus saliphilus. sp. nov., a moderately halophilic bacterium isolated from a saltern in Korea Yun Wang1 · Gang‑Qiang Jiang2 · Hong‑Ping Lin1 · Peng Sun1 · Hong‑Yan Zhang1 · Dong‑Mei Lu1 · Li‑Yun Wang1 · Chang‑Jin Kim4 · Shu‑Kun Tang3 Received: 21 July 2020 / Revised: 28 August 2020 / Accepted: 12 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel moderately halophilic bacterial strain, designated YIM 93176T, was isolated from a saltern in Korea and subjected to a polyphasic taxonomic study. This isolate YIM 93176T was observed to grow in the presence of 0–22% (w/v) NaCl and at pH 6.0–10.0 and 10–45 °C; optimum growth was observed with 5–10% (w/v) NaCl and at pH 7.0–9.0 and 28–37 °C. Based on 16S rRNA gene sequences analysis, the nearest relatives were Lentibacillus alimentarius M2024T (96.5% similarity), followed by Virgibacillus carmonensis LMG 2 0964T (96.0%) and the other type strains of the family Bacillaceae, but phylogenetic analysis indicated that strain YIM 9 3176T belonged to the cluster comprising type species of the genus Lentibacillus. Genome sequencing of strain YIM 93176T revealed a genome size of 3.2 Mb and a DNA G + C content of 40.5 mol%. The major fatty acids were anteiso-C15:0 (40.7%) and iso-C15:0 (26.4%), while the predominant respiratory quinone was menaquinone 7. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. These genotypic and chemotaxonomic characteristics supported affiliation of strain YIM 9 3176T to the genus Lentibacillus. In addition, phenotypic characteristics could distinguish strain YIM 93176T from its closely related species in genus Lentibacillus. Based on the cumulative evidences from the polyphasic taxonomic study, strain YIM 9 3176T represents a novel species of the genus Lentibacillus, for which name Lentibacillus saliphilus sp. nov. (type strain YIM 9 3176T = CCTCC AB 208139T = DSM 21375T) is proposed. Keywords Lentibacillus saliphilus sp. nov. · Saltern sediment · Polyphasic taxonomy
Introduction Communicated by Erko stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains YIM 93176T is KY697304. The draft whole genome sequence for YIM 93176T has been deposited at DDBJ/ENA/GenBank under accession number JABJXD000000000. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02043-2) contains supplementary material, which is available to authorized users. * Gang‑Qiang Jiang [email protected]
The genus Lentibacillus belongs to the family Bacillaceae of the phylum Firmicutes, was first proposed on the basis of results obtained with the 16S rRNA cataloguing technique of several closely related organisms (Yoon et al. 2002). Subsequently the description of the genus has been emended twice by Jeon et al. (2005) and Sun et al. (2016). At the time of writing, the genus contained 17 validly named species, as reported on the LSPN website (https:
Data Loading...
