Leucocytes Methods and Protocols
The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Leucocytes Methods and Protocols
Edited by
Robert B. Ashman School of Dentistry, University of Queensland, Brisbane, QLD, Australia
Editor Robert B. Ashman, Ph.D. School of Dentistry University of Queensland Brisbane, QLD, Australia [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-526-8 e-ISBN 978-1-61779-527-5 DOI 10.1007/978-1-61779-527-5 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011945167 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity, both in itself, and in its interactions with the adaptive immune system. As technology has advanced, many important questions, long thought to have been resolved, have been revisited, often yielding unexpected or novel insights. The ability to modify the genome in vivo, as in the creation of transgenic mice, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. More conventional approaches, such as flow cytometry, have also become increasingly sophisticated. This edition of “Methods in Molecular Biology” provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. It should be of use not only to the working scientist, but also to those entering the field, who appreciate the type of advice most often provided by their peers. I would like to thank the Chief Editor, Professor John Walker, for his advice, and the contributors for their cooperation and, in many cases, their forbearance, during the preparation of this book. Brisbane, QLD, Australia
Robert B. Ashman, Ph.D.
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Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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