Nocardia terrae sp. nov., an actinomycete isolated from soil in Thailand
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ORIGINAL PAPER
Nocardia terrae sp. nov., an actinomycete isolated from soil in Thailand Pawina Kanchanasin1 · Masahiro Yuki2 · Takuji Kudo2 · Moriya Ohkuma2 · Wongsakorn Phongsopitanun1 · Somboon Tanasupawat1 Received: 27 May 2020 / Revised: 19 October 2020 / Accepted: 20 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel actinomycete strain ET3-3T isolated from soil collected from Chachoengsao province, Thailand was taxonomic evaluated using a polyphasic approach. Chemotaxonomic analysis indicated that meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Mycolic acids were present. Ribose, arabinose, and galactose were detected in its whole-cell hydrolysates. The strain comprised C 16:0 and TBSA 10-methyl C 18:0 as the main fatty acids and MK-8(H4ωcycl) as the predominant menaquinone. Major polar lipids were phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, and diphosphatidylglycerol. The results of 16S rRNA gene sequence analysis showed that strain ET3-3T was closely related to Nocardia seriolae JCM 3 0082T (99.2%), Nocardia yunnanensis JCM 3 0082T (98.4%), and Nocardia concava JCM 12351T (98.2%). The draft genome of ET3-3T was 9.31 Mb with 8826 coding sequences with an average G + C content of 68.0%. The comparison of the draft genome of strain ET3-3T and N. seriolae NBRC 15557T showed the ANIb, ANIm and the digital DNA-DNA hybridization values of 86.3%, 88.5% and 32.9%, respectively. The results of the taxonomic analysis suggested that strain ET3-3T represented a novel species of the genus Nocardia for which the name Nocardia terrae sp. nov. is proposed. The type strain is ET3-3T (= JCM 33776T = TISTR 2837T). Keywords Actinomycetes · Nocardia terrae · Nocardiaceae · Soil
Introduction The genus Nocardia is the largest genus in the family Nocardiaceae of the order Corynebacteriales within the class Actinobacteria, and the type species was originally proposed by Trevisan (Trevisan 1889). At the time of writing, the genus contains 115 species with validly published names in the List of Prokaryotic Names with Standing in Nomenclature (https://www.bacterio.net/genus/nocardia). Members of this Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02107-3) contains supplementary material, which is available to authorized users. * Somboon Tanasupawat [email protected] 1
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand
Japan Collection of Microorganisms, RIKEN BioResource Center, 3‑1‑1 Koyadai, Tsukuba, Ibaraki 305‑0074, Japan
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genus can be distinguished from one another using a combination of morphological, biochemical and chemotaxonomic, characteristics (Goodfellow et al. 1999; Linos et al. 2002). Nocardia species are frequently isolated from materials collected from terrestrial and
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