Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina
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Oecophyllibacter saccharovorans gen. nov. sp. nov., a bacterial symbiont of the weaver ant Oecophylla smaragdina§ Kah-Ooi Chua1, Wah-Seng See-Too1, Jia-Yi Tan1, Sze-Looi Song2,3, Hoi-Sen Yong1, Wai-Fong Yin1, and Kok-Gan Chan1,4* 1
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia 3 China-ASEAN College of Marine Sciences, Xiamen University Malaysia, 43900 Sepang, Selangor, Malaysia 4 International Genome Centre, Jiangsu University, Zhenjiang, P. R. China (Received Jun 26, 2020 / Revised Aug 19, 2020 / Accepted Sep 10, 2020)
In this study, bacterial strains Ha5T, Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15–37°C (optimum, 28–30°C) and in the presence of 0–1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C18:1 ω7c, C16:0, C19:0 ω8c cyclo, C14:0, and C16:0 2-OH. Strains Ha5T, Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56–94.63%) with Neokomagataea tanensis NBRC106556T of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5T, Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteraceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5T as the type strain. Keywords: Oecophyllibacter saccharovorans, Acetobacteraceae, insect, phylogenomics, percentage of conserved protein *For correspondence. E-mail: [email protected] § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright ⓒ 2020, The Microbiological Society of Korea
Introduction The family Acetobacteraceae within the order Rhodospirillales consists of Gram-negative, aerobic, and non-spore forming bacteria (Gillis and De Ley, 1980; Yamada, 2016). Members of this family are known for their ability to oxidize carbohydrates and a major group of them are recognized as acetic acid bacteria (AAB) (Yamada and Yukphan, 2008
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