Planar Cell Polarity Methods and Protocols
Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols, expert researche
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Planar Cell Polarity Methods and Protocols
Edited by
Kursad Turksen Regenerative Medicine Program, Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute, Ottawa, ON, Canada
Editor Kursad Turksen, Ph.D. Regenerative Medicine Program Sprott Centre for Stem Cell Research Ottawa Hospital Research Institute Ottawa, ON, Canada [email protected]
Additional material to this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-509-1 e-ISBN 978-1-61779-510-7 DOI 10.1007/978-1-61779-510-7 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011943888 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface With the ever-increasing awareness of the importance of Planar Cell Polarity in not only normal development but also disease states, I felt that it was timely to gather a number of protocols related to this topic. I believe that this collection of protocols will be valuable to both novices and experts in the field. I thank all of the contributors for their efforts in putting together the details of their favorite protocols. This volume would not have materialized without their efforts. I would also like especially to acknowledge Dr. Peng Chen who was extremely supportive of me at the initiation of this project. As always, I am grateful to Dr. John Walker for his continuous support and for his commitment to maintaining such a high-quality series. My thanks also to Patrick Marton for his encouragements and help. Finally, a special thank you goes to David Casey for his tremendous help in the production of the volume. Ottawa, ON, Canada
Kursad Turksen, Ph.D.
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Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and D
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